Activation of carbamazepine-responsive T-cell clones with metabolically inert halogenated derivatives  John Farrell, MPhil, Maike Lichtenfels, MPhil, Andrew Sullivan, MRes, Emma C. Elliott, PhD, Ana Alfirevic, PhD, Andrew V. Stachulski, PhD, Munir Pirmohamed, FRCP, Dean J. Naisbitt, PhD, B. Kevin Park, PhD  Journal of Allergy and Clinical Immunology  Volume 132, Issue 2, Pages 493-495 (August 2013) DOI: 10.1016/j.jaci.2013.02.045 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Activation of 6 clones (4 CD4+, 2 CD8+) from a CBZ-sensitive subject with halogenated CBZ derivatives. A, Proliferation was measured by the addition of [3H]thymidine. Co-efficient of variation was less than 20%. Each color depicts a single clone. B, IFN-γ ELISpot. Journal of Allergy and Clinical Immunology 2013 132, 493-495DOI: (10.1016/j.jaci.2013.02.045) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Activation of T-cell clones (all CD8+) from a CBZ-naive subject with halogenated CBZ derivatives. Proliferation was measured by the addition of [3H]thymidine. Results show the maximum response seen with each CBZ derivative (2-bromo CBZ was not available for studies with the naive subject). Data as mean ± SD. The Mann-Whitney test was used to compare proliferation in the presence and absence of antigen. Journal of Allergy and Clinical Immunology 2013 132, 493-495DOI: (10.1016/j.jaci.2013.02.045) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions