Fig. 3. Reporter assay for the RS-ATL8 reporter cell line against shrimp extracts (A, B) RS-ATL8 cells were sensitized overnight with diluted pooled sera.

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Fig. 3. Reporter assay for the RS-ATL8 reporter cell line against shrimp extracts (A, B) RS-ATL8 cells were sensitized overnight with diluted pooled sera (1:100) from shrimp allergic patients and non-allergic healthy control serum. Cells were stimulated with (A) raw or (B) cooked shrimp extracts. Pooled allergic sera are shown by the gray bars and non-allergic healthy control serum is shown by the black bars. Data are expressed as mean±SD of the readings of triplicates. (C) The RS-ATL8 cells were sensitized overnight with individual shrimp allergic serum and stimulated with 1 µg/mL raw shrimp extract. Luminescence was measured 3 hours after stimulation. Data are expressed as mean±SD of the readings of triplicates. n.s., not significant difference; SD, standard deviation; NC, control non-allergic serum. *P<0.05. Fig. 3. Reporter assay for the RS-ATL8 reporter cell line against shrimp extracts (A, B) RS-ATL8 cells were sensitized overnight with diluted pooled sera (1:100) from shrimp allergic patients and non-allergic healthy control serum. Cells were stimulated with (A) raw or (B) cooked shrimp extracts. Pooled allergic sera are shown by the gray bars and non-allergic healthy… Allergy Asthma Immunol Res. 2018 Jan;10(1):62-76. https://doi.org/10.4168/aair.2018.10.1.62