EGFR activation in GCs inhibits GAP formation and luminal antigen delivery throughout life. EGFR activation in GCs inhibits GAP formation and luminal antigen.

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Presentation transcript:

EGFR activation in GCs inhibits GAP formation and luminal antigen delivery throughout life. EGFR activation in GCs inhibits GAP formation and luminal antigen delivery throughout life. (A) GAPs per crypt or villus cross section in the SI (blue) or colon (red) of SPF-housed mice in the presence of tropicamide (t) or CCh (c) treatment on DOL8, DOL18, or DOL28. (B) Immunofluorescence staining of phosphorylated EGFR (pEGFR; red) and cytokeratin 18 (CK18; green) in colon sections from DOL8, DOL18, and DOL28 mice; 4′,6-diamidino-2-phenylindole nuclear stain (blue). (C) Amount of phosphorylated EGFR in the colon epithelium of DOL8, DOL18, or DOL28 mice measured by ELISA. (D) GAPs per crypt (colon) or villus (SI) cross section in DOL8, DOL18, or DOL28 SPF-housed mice in the presence or absence of EGFR inhibition (EGFRi). (E) Ratio of GAPs per GC and (F) percentage of LP-MNPs staining with luminal Ova-647 in the SI and colon of DOL7, DOL14, DOL21, or DOL28 mice lacking EGFR in GCs or Cre-negative littermate controls. (G to J) Mice were treated with vehicle or inhibition of EGFR activation (EGFRi) on DOL14 and DOL16 or DOL24 and DOL26 and LP-MNPs isolated or TCR transgenic T cells adoptively transferred on DOL18 or DOL28, respectively. (G) Fold increase in CBir1 transgenic T cells after ex vivo culture with LP-MNPs from the colon isolated on DOL18 or DOL28. (H) Percentage of CD45.1+ CBir1 or DP1 cells of the total CD4+ T cells in the MLNs 3 days after transfer into recipient mice on DOL18 or DOL28. (I) Percentage of naïve (CD62L+) CBir1 or DP1 cells in the MLNs 3 days after transfer into recipient mice on DOL18 or DOL28. (J) Percentage of Foxp3+ CBir1 or DP1 cells in the colon LP 7 days after transfer into recipient mice on DOL18 or DOL28. *P < 0.05; n = 4 mice per group. Experiments in (A) to (D) and (H) were repeated three independent times, and experiments in (E) to (G), (I), and (J) were repeated two independent times. Kathryn A. Knoop et al. Sci. Immunol. 2017;2:eaao1314 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works