Cellular analysis of Epo transgenic mice.

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Cellular analysis of Epo transgenic mice. Cellular analysis ofEpotransgenic mice. (A) rHuEPO rescue in transgenic Epo mice. rHuEPO was injected at either 120 U/kg (low dose) and 1200 U/kg (high dose) into EpoWT/WT and EpoΔ/Δ mice. At 4 days after injection, whole blood was measured for reticulocytes by thiazole orange staining. Experiments were performed in triplicate. (B) Hematocrit readings of transgenic mouse after pellet implantation. EpoΔ/Δ and EpoWT/Δ, with or without the EIIa-Cre allele, were analyzed over a 140-day time course. n=6 EpoΔ/Δ; n=5 EpoWT/Δ; n=11 EpoWT/floxCreNeg. P-values on graph are a statistical comparison between EpoΔ/Δ and EpoWT/Δ. Brandon M. Zeigler et al. Dis. Model. Mech. 2010;3:763-772 ©2010 by The Company of Biologists Limited