Metabolite and carbonic anhydrase levels in Sabella spallanzanii in response to high PCO2 conditions. Metabolite and carbonic anhydrase levels in Sabella.

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Metabolite and carbonic anhydrase levels in Sabella spallanzanii in response to high PCO2 conditions. Metabolite and carbonic anhydrase levels in Sabella spallanzanii in response tohigh PCO2 conditions. (A) Histograms representing the percentage change of mean ATP, arginine, d-lactate, succinate and carbonic anhydrase of the polychaete S. spallanzanii collected from low PCO2/high pH conditions outside CO2 vents and exposed to (i) low PCO2 conditions outside the vents (control) and (ii) high PCO2 conditions within the vents (acidified). The mean biochemical parameter measured under control conditions was set as 100% and the mean biochemical parameter measured under acidified conditions was recalculated accordingly. *Significant difference between the mean biochemical parameter measured under control conditions and acidified conditions according to the generalised linear model (GLM) test (P<0.05). (B,C) The relationship between individuals' levels of the enzyme carbonic anhydrase and arginine (B) and AMP (C) in S. spallanzanii when exposed to high PCO2 (filled circles) or low PCO2 (open circles) conditions. Data points represent individual measurements. The Pearson correlation coefficient and degrees of freedom, together with the probability values (P) are provided. Regression lines are shown as solid or dashed lines denoting significant and non-significant relationships, respectively. Lucy M. Turner et al. J Exp Biol 2015;218:2148-2151 © 2015. Published by The Company of Biologists Ltd