Fig. 5. Tau phospho-epitopes phosphorylated by human GSK3β in the Drosophila visual system.Sarcosyl-soluble and -insoluble Tau fractions were purified from fly heads overexpressing 2N4R Tau from the 68A locus with or without hGSK3β. Tau phospho-epitopes phosphorylated by human GSK3β in the Drosophila visual system.Sarcosyl-soluble and -insoluble Tau fractions were purified from fly heads overexpressing 2N4R Tau from the 68A locus with or without hGSK3β. Four phospho-specific antibodies were used to identify specific phosphorylation events mediated by hGSK3β in vitro. Giulia Povellato et al. Biology Open 2014;3:1-11 © 2013. Published by The Company of Biologists Ltd