Validation of select PDN drug candidates in an in vivo endotoxemia model Validation of select PDN drug candidates in an in vivo endotoxemia model Therapeutic.

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Validation of select PDN drug candidates in an in vivo endotoxemia model Validation of select PDN drug candidates in an in vivo endotoxemia model Therapeutic leads generated using PDN were directly tested for survival benefit using a murine model of endotoxemia. Select compounds were injected 24 h before and on the day of LPS administration, using routes and doses specified in the methods. C57bl/6 female mice were injected with a high‐lethality dose of Escherichia coli LPS (38–40 μg/g) followed by a subcutaneous injection of sterile saline. Significant differences in concentration between drug and vehicle‐treated pre‐ and post‐pubertal mice are labeled with ****P < 0.0001, ***P < 0.001, **P < 0.01, or *P < 0.05. Percent survival was compared using a log‐rank Mantel–Cox test. Rose B Joachim et al. Mol Syst Biol 2018;14:e7998 © as stated in the article, figure or figure legend