KIF18A sNL variants accumulate as well as WT on peripheral K-fibers.

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KIF18A sNL variants accumulate as well as WT on peripheral K-fibers. KIF18A sNL variants accumulate as well as WT on peripheral K-fibers. (A) Representative metaphase-arrested cells expressing GFP-WT KIF18A or sNL1-3 variants. Shown is one z-slice near the top of the spindle. Blue arrows highlight example peripheral K-fibers. (B) Quantification of KIF18A accumulation on peripheral K-fiber ends by line scan analysis. Individual lines were normalized and aligned to peak ACA intensity. Symbols show average; error bars are SD. Red, ACA; blue, α-tubulin; and green, KIF18A variant. No significance found by F test comparing slopes of linear regressions for WT versus any sNL variant. Data obtained from two independent experiments with the following cell and line numbers: GFP-WT (13 cells, 73 lines), GFP-sNL1 (17 cells, 86 lines), GFP-sNL2 (15 cells, 76 lines), and GFP-sNL3 (20 cells, 102 lines). Source data are available for this figure. Heidi LH Malaby et al. LSA 2019;2:e201800169 © 2019 Malaby et al.