Fig. 7. Vinculin recruitment enhances the efficiency of barrier formation.(A) TER measurements after a calcium switch in α-catenin-depleted MDCK cells.

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Scanning electron microscopy analysis of EGK-I to -V chick embryos.

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Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

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Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

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Fig. 7. Vinculin recruitment enhances the efficiency of barrier formation.(A) TER measurements after a calcium switch in α-catenin-depleted MDCK cells rescued with the indicated constructs. Vinculin recruitment enhances the efficiency of barrier formation.(A) TER measurements after a calcium switch in α-catenin-depleted MDCK cells rescued with the indicated constructs. Error bars indicate standard error, n>3. (B) TER measurements after a calcium switch in α-catenin-depleted MDCK cells rescued with Ecad-αcat fusions that contain α-catenin-WT or α-catenin-ΔVBS show that mechanosensing at the Cadherin complex is sufficient for force-enhanced barrier formation. Error bars indicate standard error, n>3. (C) IF images of α-catenin-depleted MDCK cells, rescued with α-catenin-WT or α-catenin-ΔVBS, fixed at 3 hours after calcium showing the delayed maturation into AJs when vinculin cannot be recruited. Scale bar = 10 µm. Floor Twiss et al. Biology Open 2012;bio.20122428 © 2012. Published by The Company of Biologists Ltd