Retinoic acid sentinels in live embryos and responding to exogenous RA in half-embryo cultures. Retinoic acid sentinels in live embryos and responding.

Slides:

Advertisements

Similar presentations

Mitochondria localize to both the front and the rear of neutrophils.

Advertisements

SESN2 expression in lungs from healthy donors, habitual smokers without COPD and individuals with late-stage COPD. (A) Representative western blots of.

Change in fluorescence / min

Obesity accompanied by increased triacylglyceride deposits in InsP3R mutants leads to starvation resistance. Obesity accompanied by increased triacylglyceride.

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Visualisation of metabolic tissues in zebrafish.

Fig. 3. HECTD2 contains a naturally occurring polymorphism at A19, which mislocalizes to the cytosol. HECTD2 contains a naturally occurring polymorphism.

Inducible DM1 model displays increased autophagy.

Inhibition of PI3K or SERCA phenocopies polycystin loss of function.

Differential Cre-ERT activity and PTEN deletion in endometrial epithelial and stromal compartments. Differential Cre-ERT activity and PTEN deletion in.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle

Alterations in Notch signaling affect the frequency of intermediate and main IHBD branches. Alterations in Notch signaling affect the frequency of intermediate.

Fig. 4. A primary screen based on scrape closure

A Drosophila cell culture model to study VAP(P58S) aggregation.

BAC recombineering, gene targeting and RMCE strategies.

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

Altered palate and jaw morphology in LgDel mice.

MicroCT analysis reveals that IHBD average volume is modulated with age upon deletion of RBP-J or activation of Notch1. MicroCT analysis reveals that IHBD.

Tbx1 regulates the Mef2c AHF enhancer in vivo.

OCT and IOP analysis of 2-3-month-old AP-2β NCC KO mutants.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Fig. 5. Onecut transcription factors are important for the correct generation of the mdDA neuronal population.(A) Schematic representation of the region.

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

Fig. 2. Salivary glands from RasV12-expressing larvae produce MMP1, release tissue fragments into the hemolymph and express apoptotic markers.Salivary.

Survival and telomere length of G2tert−/− zebrafish in a p53−/− background. Survival and telomere length of G2tert−/− zebrafish in a p53−/− background.

Hypoacidity, anemia and hypergastrinemia in KI/KI mice.

Mutation in pycr1a exon 3 disrupts predicted exonic splicing enhancers

Expression data confirm and extend existing knowledge

Combined treatment with LY and sunitinib rescued hypervascularization.

Dose-responsiveness and specificity of retinoic acid signaling sentinels. Dose-responsiveness and specificity of retinoic acid signaling sentinels. (A)

Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. Effects of doxorubicin.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 2. Blocking of BMP signaling, but not of nodal signaling, inhibits autochthonous neural crest migration in zebrafish embryos. Blocking of BMP signaling,

Fig. 7. Lhx1-RNAi reduces the eye size

The TER94-p47 complex isinvolved in Notch signaling regulation

Integrin binding of soluble GRGDSP and fibronectin.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Simultaneous perturbations to shuttling result in defects in the Dl gradient and in the sna domain. Simultaneous perturbations to shuttling result in defects.

The effect of TDP-43A315T expression on dendrite spine development.

Fusion of PALB2(L21P) to the BRCT repeats of BRCA1 mediates the assembly of DNA damage foci by PALB2 and RAD51 in PALB2-deficient cells. Fusion of PALB2(L21P)

BMP signalling is dispensable for early gastruloid patterning.

Distribution and extent of expression.

Chemical chaperone 4-PBA rescues the hi559 GI phenotype.

prdm1a is co-expressed with foxd3 and tfap2a at the NPB

Reb does not play a significant role in regulating TGF-β signaling

Chronic phenytoin increases activity levels in Mecp2Null/Y mice.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Fig. 1. Phenotypes of RasV12 transformed Drosophila lines

Homozygous col22a1−/− embryos show increased percentage of hemorrhages when raised at increased temperature. Homozygous col22a1−/− embryos show increased.

Effect of the plastid translation inhibitor Spec on LR development

Presence of CGH-1 in the embryo.

Fig. 3. Exogenous folic acid rescues neural epithelial apical constriction and activation of non-muscle myosin upon Rho-kinase inhibition. Exogenous folic.

Two domains of KHC act to position the nucleus.

Folic acid increases apical junctional pMLCK localization in vivo

HEK-cFXN cells show a correlation among ROS levels, LIP and frataxin expression. HEK-cFXN cells show a correlation among ROS levels, LIP and frataxin expression.

MMP-9 expression. MMP-9 expression. (A) Lungs from M. tuberculosis-infected (H37Rv, 14 wpi, untreated) and age-matched uninfected (control) mice were harvested.

Ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis. ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis.

Association of MMP expression with macrophages.

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

The zebrafish swdp75fm and swdp82mf mutations cause exocrine pancreatic hypoplasia and reduced skin pigmentation. The zebrafish swdp75fm and swdp82mf mutations.

Fig. 2. Expression of Cx43 mutant T154A resulted in non-radial spreading and formation of protrusions in J558µm3 cells spreading in response to BCR signaling.(A)

Altered intracellular distributions of acetylated α-tubulin, mitochondria and peroxisomes. Altered intracellular distributions of acetylated α-tubulin,

Peroxisome speeds were slower in patient and control cells.

Fig. 1. Cell adhesion molecule expression and the aggregation of wildtype and mutant ES cells.(A) Wildtype (WT), E-cadherin null (9J), and N-cadherin null.

Small molecule suppression of ATXN3-CAG89 aggregation.

Fig. 1. Expression of a Ror-eGFP fusion protein under control of the endogenous Ror promoter in Drosophila embryos. Expression of a Ror-eGFP fusion protein.

Fig. 7. Rho family GTPase signaling is required for cyst formation in Ptp4E Ptp10D mutants.(A–C) Expression of DN Rho1 and Rac1 mutants in wild-type (w1118)

Histone crotonylation in kidney tubular cells.

2DG suppresses of lamellipodia and filopodia and causes disorganization of F-actin filaments in murine endothelial cells. 2DG suppresses of lamellipodia.

Presentation transcript:

Retinoic acid sentinels in live embryos and responding to exogenous RA in half-embryo cultures. Retinoic acid sentinels in live embryos and responding to exogenous RA in half-embryo cultures. (A) Merged bright field and fluorescence images of live E8.25 and E9.5 embryos containing the retinoic response sentinel DR5-TA-CFP at the −60 and −228 sites of the endogenous Rosa26 promoter. Composite images were generated with Photoshop. Fluorescent domains are noted. (B) Half-embryo fragments from E8.5 embryos of the designated genotypes were cultured overnight with 1 μM RA or DMSO carrier (control). Images are shown before (left) and after (right) culture, revealing an anterior-ward extension of the fluorescent signal upon RA treatment for each construct (white arrows). In supplementary material Fig. S3, data are shown indicating that only RA extended the CFP expression domain anteriorly, and not the RA inhibitor AGN193109, BMP4, noggin, a TGFβ inhibitor or a MAPK inhibitor, demonstrating specificity. Palle Serup et al. Dis. Model. Mech. 2012;5:956-966 © 2012. Published by The Company of Biologists Ltd