The human homolog of clu, CLUH, can rescue Drosophila clu mutant phenotypes. The human homolog of clu, CLUH, can rescue Drosophila clu mutant phenotypes.

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The human homolog of clu, CLUH, can rescue Drosophila clu mutant phenotypes. The human homolog of clu, CLUH, can rescue Drosophila clu mutant phenotypes. (A) Schematic of Drosophila and human Clu/CLUH. In silico analysis predicts several domains. Clu, Clu domain; TPR, tetratricopeptide repeats; DUF, domain of unknown function; β-GF, beta grasp fold; ms, melanogaster specific. (B-E) S2R+ cells normally have dispersed mitochondria (B, green). Upon clu RNAi treatment, mitochondria become tightly clumped (C, green, arrow). The mitochondrial clumping (D,E, arrows) is rescued by expressing either Drosophila full-length Clu (D, magenta, arrowheads) or CLUH (E, magenta, arrowheads). Dotted lines outline cells. (F) Quantification of B-E. See Materials and Methods for details. (G) A western blot demonstrating that clu RNAi treatment effectively eliminates Clu protein. (H-K) Female germ cells labeled with mitochondria (green) and membranes (magenta) to outline and mark the somatic cells. Mitochondria in wild-type germ cells (H, green) are dispersed in the germplasm (dotted line). Germ cells lacking Clu (I, dotted line) have clumped mitochondria (green, arrow). Overexpressing either Drosophila full-length Clu (J) or CLUH (K) disperses mitochondria compared with in the clu mutant. (L) Expressing Clu or CLUH in a clu-null background completely rescues the sterility of clu-null mutant females. Green=α-CVA, magenta=α-GFP (D,E), α-1B1 (hu li tai shao, adducin-like protein) (H-K), blue=DAPI. Scale bars: 10 μm in E for B-E, in K for H-K. Aditya Sen et al. Dis. Model. Mech. 2015;8:577-589 © 2015. Published by The Company of Biologists Ltd