Gene expression is reduced when RNAP binds a promoter too tightly.

Slides:

Advertisements

Similar presentations

20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.

Advertisements

Modelling the stability of Stx lysogens

Transcriptional profiling I – microarrays and proteomics

GenePolypeptide Gene  Polypeptide Transcription 1.RNAP binds to promoter 2.Separates DNA strands 3.Transcribes the DNA (adds RNA nucleotides in a 5'-3'

Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein.

Observation vs. Inferences The Local Environment.

Gene Expression Chapter 16. DNA regulatory sequence All on DNA Promoters – Start transcription Promoters – Start transcription Terminators – End Transcription.

Noise and Variability in Gene Expression Presenting: Tal Ashuah Advisor: Dr. Alon Zaslaver 10/05/15.

Supplementary figure 5.

Alignment table: group 4

Control of Gene Expression

DNA, RNA and Genes Science 10 – 2017/18 Mrs. Day.

Regulation of Gene Expression by Eukaryotes

Hydrogen solubility isotherms and solute susceptibility.

Comparison (θ) of the mean dinucleotide variability distribution for (A) genes with MAE vs. Comparison (θ) of the mean dinucleotide variability distribution.

Comparison (R) of the mean observed to expected dinucleotide distribution for (A) genes with MAE vs. Comparison (R) of the mean observed to expected dinucleotide.

Balancing selection characterizes both MAE genes and cell type-specific genes (expressed in either nsc or asl but not in both). Balancing selection characterizes.

(A) Heat map showing expression changes during in vitro differentiation of nscs to astrocytes. (A) Heat map showing expression changes during in vitro.

Distribution of mutations in different groups of genes, in cancer with extreme ML values. Distribution of mutations in different groups of genes, in cancer.

Empirical mutation-selection phase diagram of tumor evolution.

Comparison of Cg-OxyR active-site pocket with previously published structures. Comparison of Cg-OxyR active-site pocket with previously published structures.

Transient overexpression of FveCYP707A4a delayed fruit ripening.

Dysregulation of Wnt/β-catenin and EphB/EphrinB signaling after 56Fe radiation. Dysregulation of Wnt/β-catenin and EphB/EphrinB signaling after 56Fe radiation.

The evolution of WCI for biological specificity makes organisms more evolvable. The evolution of WCI for biological specificity makes organisms more evolvable.

Behaviorally dependent allele-specific expression.

Auxin and GA promote fruit growth by activating ABA catabolism in the early stage and are important for the regulation of interlinked regulatory loops.

The inferred metabolism in Sulcia and Nasuia bacteriocytes.

Cg-OxyR controls catalase transcription, recovery from lag phase, survival to H2O2 stress, and H2O2 scavenging rate. Cg-OxyR controls catalase transcription,

Two functional states of human CFTR

Schematic of elephant ivory showing longitudinal cross-section of a tusk with pulp cavity, dentine, and cementum. Schematic of elephant ivory showing longitudinal.

Representation of the evolutionary model.

Cg-OxyR oxidation decreases binding affinity and extension to the catalase promoter region. Cg-OxyR oxidation decreases binding affinity and extension.

Cell-type–specific ERV expression in primary cells.

Conformational changes of the ion-permeation pathway.

WCI evolve as organisms become more complex.

Patients with SLE have elevated ERV expression.

Estimation of R(t). Estimation of R(t). (A) Daily R(t) as inferred from the daily incidence of new infections for one stochastic model realization. Tg.

Motion strength and duration influence both motion direction choices and environment choices. Motion strength and duration influence both motion direction.

Vegfa is overexpressed by E11

Increased switch bound explains reduced switch rates for longer environments. Increased switch bound explains reduced switch rates for longer environments.

Long-term up-regulation of proliferative markers and down-regulation of cell migration factors along with increased intestinal metaplasia after 56Fe radiation.

Transcriptional response to mating in whole A. gambiae females.

Host transcriptional profiling distinguishes patients with acute LRTI (LRTI+C+M) from those with noninfectious acute respiratory illness (no-LRTI). Host.

RNA-seq analysis of iWAT and eWAT.

Inferred tree for species in the genus Solanum (S. ) labeled with HRFs

Combined LRTI prediction metric integrating pathogen detection and host gene expression. Combined LRTI prediction metric integrating pathogen detection.

Structural overview. Structural overview. (A) Domain organization of LRRK2. The residue boundary of the WD40 domain and locations of three recurrent disease.

PCA of RNAseq data from CTL TB and EOPE TB (GSE119265).

Volume 6, Issue 4, Pages e10 (April 2018)

Expression of NCoR1∆ID in PAX8−/− mice does not reverse repression of T3-positive target genes. qPCR on the indicated pituitary (A) and neuronal (B) genes.

GC analysis of V4 feedback modulations based on LFP data.

Volume 91, Issue 12, Pages (December 2006)

Prokaryotic (Bacterial) Gene Regulation

DNA, RNA and Genes Science 10 – 2017/18 Mrs. Day.

Pyoverdine production measured as RFUs standardized by OD significantly decreases with length of infection in years. Pyoverdine production measured as.

The tetracycline-regulated female-killing system.

(Upper) Blind-to-controls generalization performance: prediction accuracies observed when the classifier was trained on the auditory trials from the blind.

NsiR4 expression is mediated through an NtcA-activated promoter.

Schematic representation of our random walk model with environmental state-dependent dynamics. Schematic representation of our random walk model with environmental.

(A–F) FRET provides evidence for compaction.

BHLH1a localizes in the nucleus, and its overexpression alters rhythmic diel gene expression. bHLH1a localizes in the nucleus, and its overexpression alters.

SDT-MIX simulated relative efficiencies for the search task.

Expression of promoters with no UP element.

Effect of pH and bicarbonate (HCO3) vs

The bivalent nature of RNAP–promoter binding.

Fig. 4 Mutations and reduced expression of human KDM6A predict poor prognosis of female patients with BCa. Mutations and reduced expression of human KDM6A.

Schematic representation of the involvement of each of the four genes on virulence factor expression. Schematic representation of the involvement of each.

Theory in Biology: Figure 1 or Figure 7?

Confirmation of candidates from WT1 binding site screen.

Protein Synthesis.

Presentation transcript:

Gene expression is reduced when RNAP binds a promoter too tightly. Gene expression is reduced when RNAP binds a promoter too tightly. Shown is measured expression vs. inferred promoter strength −ΔERNAP (stronger promoters on the right). Expression decreases once the RNAP binding becomes comparable to the free energy of the transcription initiation state −ΔEtrans=6.2 kBT. The dashed line shows the prediction of the multivalent model. Tal Einav, and Rob Phillips PNAS doi:10.1073/pnas.1905615116 ©2019 by National Academy of Sciences