Parathyroid hormone (1-34) prevents cartilage degradation and preserves subchondral bone micro-architecture in guinea pigs with spontaneous osteoarthritis

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Parathyroid hormone (1-34) prevents cartilage degradation and preserves subchondral bone micro-architecture in guinea pigs with spontaneous osteoarthritis J.-y. Yan, F.-m. Tian, W.-Y. Wang, Y. Cheng, H.-P. Song, Y.-Z. Zhang, L. Zhang Osteoarthritis and Cartilage Volume 22, Issue 11, Pages 1869-1877 (November 2014) DOI: 10.1016/j.joca.2014.07.013 Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Outline of the proximal tibia and histological findings of articular cartilage at distal femur by Masson staining (×200) and OARSI score of the articular cartilage in each group. A, 1 month group; B, 3 months group; C, 6 months group; D, 9 months group; E, 6 months (PTH) group; F, 9 months (PTH) group. *P < 0.05, compared with 1 month group; #P < 0.05, compared with 3 months group; &P < 0.05, compared with 6 months group; ▲P < 0.05, PTH group compared with control group. Osteoarthritis and Cartilage 2014 22, 1869-1877DOI: (10.1016/j.joca.2014.07.013) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Immunohistochemistry assay for type-II collagen and the changes of IOD in expression. (×200) Immunohistochemical analysis showed that the protein levels of type-II collagen in control group were weaker than those in the PTH treated group. A, 1 month group; B, 3 months group; C, 6 months group; D, 9 months group; E, 6 months (PTH) group; F, 9 months (PTH) group; G, negative control staining. *P < 0.05, compared with 1 month group; #P < 0.05, compared with 3 months group; &P < 0.05, compared with 6 months group; ▲P < 0.05, PTH group compared with control group. Osteoarthritis and Cartilage 2014 22, 1869-1877DOI: (10.1016/j.joca.2014.07.013) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Immunohistochemistry assay for PTH1R in the cartilage and subchondral bone and the changes of IOD in expression. (×200) Immunohistochemical analysis showed that the protein levels of PTH1R in cartilage and subchondral bone in control group were weaker than those in the PTH treated group. A, 1 month group; B, 3 months group; C, 6 months group; D, 9 months group; E, 6 months (PTH) group; F, 9 months (PTH) group; G, negative control staining. *P < 0.05, compared with 1 month group; #P < 0.05, compared with 3 months group; &P < 0.05, compared with 6 months group; ▲P < 0.05, PTH group compared with control group. Osteoarthritis and Cartilage 2014 22, 1869-1877DOI: (10.1016/j.joca.2014.07.013) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Immunohistochemistry assay for MMP-13 and SOST and the changes of IOD in expression (×200). Immunohistochemical analysis showed that the protein levels of MMP-13 and SOST in control group were stronger than those in PTH treated group. A, 1 month group; B, 3 months group; C, 6 months group; D, 9 months group; E, 6 months (PTH) group; F, 9 months (PTH) group; G, negative control staining. *P < 0.05, compared with 1 month group; #P < 0.05, compared with 3 months group; &P < 0.05, compared with 6 months group; ▲P < 0.05, PTH group compared with control group. Osteoarthritis and Cartilage 2014 22, 1869-1877DOI: (10.1016/j.joca.2014.07.013) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Immunohistochemistry assay for OPG in the cartilage and subchondral bone and the changes of IOD in expression. (×200) Immunohistochemical analysis showed that the protein levels of OPG in cartilage and subchondral bone in control group were weaker than those in the PTH treated group. A, 1 month group; B, 3months group; C, 6 months group; D, 9 months group; E, 6 months (PTH) group; F, 9 months (PTH) group; G, negative control staining. *P < 0.05, compared with 1 month group; #P < 0.05, compared with 3 months group; &P < 0.05, compared with 6 months group; ▲P < 0.05, PTH group compared with control group. Osteoarthritis and Cartilage 2014 22, 1869-1877DOI: (10.1016/j.joca.2014.07.013) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Immunohistochemistry assay for RANKL in the cartilage and subchondral bone and the changes of IOD in expression. (×200) Immunohistochemical analysis showed that the protein levels of RANKL in cartilage and subchondral bone in control group were stronger than those in the PTH treated group. A, 1 month group; B, 3 months group; C, 6 months group; D, 9 months group; E, 6 months (PTH) group; F, 9 months (PTH) group; G, negative control staining. *P < 0.05, compared with 1 month group; #P < 0.05, compared with 3 months group; &P < 0.05, compared with 6 months group; ▲P < 0.05, PTH group compared with control group. Osteoarthritis and Cartilage 2014 22, 1869-1877DOI: (10.1016/j.joca.2014.07.013) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 The ratio of OPG/RANKL expression in the cartilage and subchondral bone. *P < 0.05, compared with 1 month group; #P < 0.05, compared with 3 months group; &P < 0.05, compared with 6 months group; ▲P < 0.05, PTH group compared with control group. Osteoarthritis and Cartilage 2014 22, 1869-1877DOI: (10.1016/j.joca.2014.07.013) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions