Fig. 8. CCA and ChQ treatment induce accumulation of F-actin rings.

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Fig. 8. CCA and ChQ treatment induce accumulation of F-actin rings. CCA and ChQ treatment induce accumulation of F-actin rings. (A) PC-3 cells were fixed after a 24 h (top) or 48 h (bottom) incubation with vehicle control media (DMSO 0.005%), 5 nM of CCA, or 50 µM of ChQ, and then immunostained with phalloidin (green). DAPI (blue) was used as nuclear marker. White arrows show F-actin rings. White arrowheads show filopodial projections. Scale bar: 10 µm. (B) F-actin rings were measured in several microscope pictures and the percentage of cells with F-actin rings counted. n≥37 cells, **P<0.01, ***P<0.001, Mann-Whitney test; mean±s.e.m. Yamhilette Licon-Munoz et al. Biology Open 2017;6:1734-1744 © 2017. Published by The Company of Biologists Ltd