Fig. 5. Effect of gravity and stage on relative expression levels of molecular core clock and clock regulatory genes.Total RNA was isolated from (A) mammary.

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Fig. 5. Effect of gravity and stage on relative expression levels of molecular core clock and clock regulatory genes.Total RNA was isolated from (A) mammary and (B) liver of late pregnant (G20) and early lactation (P1) control and HG treated dams. Effect of gravity and stage on relative expression levels of molecular core clock and clock regulatory genes.Total RNA was isolated from (A) mammary and (B) liver of late pregnant (G20) and early lactation (P1) control and HG treated dams. Expression of Arntl, Npas2, Rora, Bhlh40e, Csnk1e, and Nr1d1 were measured using rt-q-PCR. Actb and B2m were used as reference genes in mammary and liver, respectively, and relative gene expression (RQ) was calculated using the delta-delta CT method with mean G20 control delta CT as normalizer. Values are expressed as mean ±SEM; to determine probability of difference, ANOVA was used with gravity, day and gravity × day as main effects; *indicates significant difference between treatments at p<0.05. Theresa Casey et al. Biology Open 2012;bio.2012687 © 2012 The Company of Biologists