Representative flow cytometric analysis of bone marrow, spleen and whole blood from EpoWT/WT, EpoΔ/Δ and EpoWT/Δ mice. Representative flow cytometric analysis of bone marrow, spleen and whole blood fromEpoWT/WT,EpoΔ/ΔandEpoWT/Δmice. Tissue was collected, processed through a 40-μm strainer, and stained for anti-CD71 and -Ter119 antibodies for 45 minutes. nexperiments=4. Whole blood was diluted and stained for thiazole orange for 30 minutes, then analyzed on BD FACS Caliber. nexperiments=4. Brandon M. Zeigler et al. Dis. Model. Mech. 2010;3:763-772 ©2010 by The Company of Biologists Limited