Histochemical assessment of nitric oxide synthase activity in aortic endothelial cells of streptozotocin-induced diabetic rats M Shafiei, M Nobakht,

Slides:

Advertisements

Similar presentations

Regular endothelial function mainly depends on the capacity to produce nitric oxide (NO). Endothelial dysfunction has been defined as the change in.

Advertisements

Nitric Oxide Synthase Cody A Scholtens CHE /21/14.

Volume 13, Issue 4, Pages (December 2006)

Contribution of nitric oxide synthase (NOS) in blood–brain barrier disruption during acute focal cerebral ischemia in normal rat Mohammad T. Mohammadi,

Cyril H. Barton, Zehnmin Ni, Nosratola D. Vaziri Kidney International

Growth hormone–releasing peptide ghrelin inhibits homocysteine-induced endothelial dysfunction in porcine coronary arteries and human endothelial cells

Schwann cell injuries of radial nerve after lead (Pb) exposure in rats

The role of lymphatic vessels in the heart

Garlic extract reduces serum angiotensin converting enzyme (ACE) activity in nondiabetic and streptozotocin-diabetic rats Mahmoud Hosseini, S. Mohammad.

Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats Mehrdad Roghani, Tourandokht.

David G. Cable, MD, James A. Caccitolo, MD, Eric A

I. Marie, Dr., J.L. Bény Journal of Investigative Dermatology

Volume 62, Issue 2, Pages (August 2002)

Volume 21, Issue 3, Pages (September 2014)

Amelioration of pancreatic and renal derangements in streptozotocin-induced diabetic rats by polyphenol extracts of Ginger (Zingiber officinale) rhizome

Edward A. Black, Tomasz J. Guzik, MD, Nick E. J

Suppression of Graft Spasm by the Particulate Guanylyl Cyclase Activator in Coronary Bypass Surgery Takeshi Kinoshita, MD, PhD, Masashi Tawa, PhD, Tomoaki.

Volume 13, Issue 4, Pages (December 2006)

Volume 19, Issue 3, Pages (June 2012)

Adriamycin-induced oxidative stress, activation of MAP kinases and apoptosis in isolated cardiomyocytes Huiquan Lou, Kuljeet Kaur, Anita K. Sharma, Pawan.

Volume 13, Issue 4, Pages (December 2006)

D. Neil Granger, Peter R. Kvietys Pathophysiology

Ontogeny of pulmonary cyclooxygenase-1 (COX-1) and -2 (COX-2)

Simvastatin pretreatment enhances ischemia-induced neovascularization and blood flow recovery in streptozotocin-treated mice Po-Hsun Huang, MD, PhD,

D. A. Lewis, PhD, R. C. Lowell, MD, R. A. Cambria, MD, P. C

Increased magnesium intake prevents hyperlipidemia and insulin resistance and reduces lipid peroxidation in fructose-fed rats L.A. Olatunji, A.O. Soladoye

Hao G. Nguyen, MD, PhD, Amit Korach, MD, Chey Collura, MA, Benjamin R

Expression and Function of Endothelial Nitric Oxide Synthase Messenger RNA and Protein Are Higher in Internal Mammary Than in Radial Arteries Guo-Wei.

Endothelium-dependent vasorelaxations in response to aggregating platelets are impaired in reversed vein grafts Kimihiro Komori, MD, Peter Gloviczki,

Volume 23, Issue 4, Pages (December 2016)

Potassium Channel-Related Relaxation by Levosimendan in the Human Internal Mammary Artery Oguzhan Yildiz, MD, PhD, Melik Seyrek, MD, Vedat Yildirim,

Calcitonin and Omega–3 Fatty Acids Exhibit Antagonistic and Non-Additive Effects in Experimental Diabetes Wale Johnson Adeyemi, Luqman Aribidesi Olayaki

Volume 22, Issue 4, Pages (December 2015)

Volume 87, Issue 1, Pages (January 2015)

Effect of short term treatment of L-carnitine on tissue ACE activity in streptozotocin- induced diabetic rats Ali M. Sharifi, Behnoush Zare, Morteza Keshavarz,

Annette Ebner, PhD, David M. Poitz, PhD, Antje Augstein, PhD, Ruth H

Volume 16, Issue 1, Pages 9-13 (June 2009)

Volume 61, Issue 1, Pages (January 2002)

Antihyperglycemic and antihyperlipidemic effect of Rumex patientia seed preparation in streptozotocin-diabetic rats Reza Sedaghat, Mehrdad Roghani, Maedeh.

Volume 18, Issue 4, Pages (September 2011)

Catherine J. Hunter, Isabelle G. De Plaen Pathophysiology

The effects of ginsenoside Rb1 on endothelial damage and ghrelin expression induced by hyperhomocysteine Zhiwei Xu, PhD, Taohua Lan, MD, Weikang Wu,

Volume 18, Issue 4, Pages (September 2011)

Volume 58, Issue 2, Pages (August 2000)

Volume 9, Issue 4, Pages (September 2003)

Thrombolysis for experimental deep venous thrombosis maintains valvular competence and vasoreactivity Jeffrey M. Rhodes, MD, Jae-Sung Cho, MD, Peter.

Nilima Shukla, PhD, Gianni D. Angelini, FRCS, Jamie Y. Jeremy, PhD

Volume 17, Issue 1, Pages (February 2010)

Experimental maternal treatment with dexamethasone during lactation induces neonatal testicular and epididymal oxidative stress; Implications for early.

Oral contraceptive administration aggravates nitric oxide synthesis inhibition-induced high blood pressure in female rats L.A. Olatunji, A.O. Soladoye

Role of the endothelium in the vascular effects of vitamin C in rats

Endothelial Nitric Oxide Synthase in Bicuspid Aortic Valve Disease

Volume 75, Issue 7, Pages (April 2009)

Effects of NADPH oxidase inhibitor in diabetic nephropathy

Volume 60, Issue 6, Pages (December 2001)

Volume 119, Issue 1, Pages (July 2000)

Volume 81, Issue 8, Pages (April 2012)

The effects of diethyldithiocarbamate, a SOD inhibitor, on endothelial function in sedentary and exercised db/db mice M. Khazaei, F. Moien-Afshari, S.

The effect of maintenance and reversal of DOCA-Salt hypertension on extravasation of macromolecules and serum nitric oxide concentration in male rats

Volume 60, Issue 1, Pages (July 2001)

Volume 75, Issue 3, Pages (February 2009)

Changes in the cooling rate and medium improve the vascular function in cryopreserved porcine femoral arteries Montserrat Rigol, PhD, Magda Heras, MD,

Volume 16, Issue 1, Pages (June 2009)

Effects of mifepristone on expression of endothelial nitric oxide synthase in human endometrium during the implantation phase Xiaoxi Sun, M.D., Xiaoyan.

Effect of Vein Graft Harvesting on Endothelial Nitric Oxide Synthase and Nitric Oxide Production Michael R. Dashwood, PhD, Kay Savage, PhD, Audrey Dooley,

Volume 57, Issue 6, Pages (June 2000)

Volume 61, Issue 3, Pages (March 2002)

Medroxyprogesterone acetate does not antagonize estrogen-induced increases in endothelium-dependent vasodilation: potential clinical implications Hillary.

Protamine induces vasorelaxation of human internal thoracic artery by endothelial NO- synthase pathway Dmitry Pevni, MD, Jacob Gurevich, MD, Inna Frolkis,

Panagiotis Kougias, MD, Hong Chai, MD, PhD, Peter H. Lin, MD, Alan B

Presentation transcript:

Histochemical assessment of nitric oxide synthase activity in aortic endothelial cells of streptozotocin-induced diabetic rats M Shafiei, M Nobakht, M Fattahi, L Kohneh-Shahri, M Mahmoudian Pathophysiology Volume 10, Issue 1, Pages 63-67 (December 2003) DOI: 10.1016/j.pathophys.2003.08.001

Fig. 1 Concentration-dependent relaxation to the endothelium-dependent vasodilator acetylcholine in control (n=8) and diabetic (n=8) aortic rings precontracted with submaximal concentrations of phenylephrine. Values are expressed as the mean±S.E.M. (*P<0.01). Pathophysiology 2003 10, 63-67DOI: (10.1016/j.pathophys.2003.08.001)

Fig. 2 Concentration-dependent relaxation to isosorbide dinitrate in control and diabetic aortic rings with endothelium ((a), n=8) or without endothelium ((b), n=8) precontracted with submaximal concentrations of phenylephrine. Values are expressed as the mean±S.E.M. Pathophysiology 2003 10, 63-67DOI: (10.1016/j.pathophys.2003.08.001)

Fig. 3 Light micrograph of aorta obtained from healthy rat. No staining could be detected in control sections after omitting βNADP in NADPH-diaphorase staining. Pathophysiology 2003 10, 63-67DOI: (10.1016/j.pathophys.2003.08.001)

Fig. 4 Light micrograph of aorta obtained from healthy rat. NADPH-diaphorase histochemistry reveals eNOS activity in endothelial cells that strongly are stained. Pathophysiology 2003 10, 63-67DOI: (10.1016/j.pathophys.2003.08.001)

Fig. 5 Light micrograph of aorta obtained from diabetic rat. Endothelial cells show much less staining for NADPH-diaphorase. Arrows point to the sites of disruption in endothelial cell membranes. Pathophysiology 2003 10, 63-67DOI: (10.1016/j.pathophys.2003.08.001)