VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown AZ-521 cells. VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown.

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VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown AZ-521 cells. VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown AZ-521 cells. (A) Verification of knockdown cells by immunoblotting (IB). Signals were generated using anti-RPTPα antibody. α-tubulin served as a loading control. Arrowhead represents RPTPα. WT, wild-type AZ-521 cells; KD, RPTPα constitutive-knockdown AZ-521 cells. (B) Detection of phospho-Tyr418 Src in knockdown cells. Cells were incubated with VacA or iVacA at 37°C, 5% CO2 for the indicated times. Signals were generated using anti-phospho-Src(Tyr418) and anti-Src antibodies, and phospho-Src signal intensity was normalized to total Src. Data were analyzed by two-tailed Student's t-test. Results are representative of five independent experiments and data are means±s.d. values from triplicate experiments with an n=5 per experiment. *P<0.01 (vs t=0). IB, immunoblotting. Masayuki Nakano et al. Dis. Model. Mech. 2016;9:1473-1481 © 2016. Published by The Company of Biologists Ltd