Fig. 4. E-cadherin expression level affects monomer dynamics.

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Fig. 4. E-cadherin expression level affects monomer dynamics. E-cadherin expression level affects monomer dynamics. (A,B) Estimation of junctional integrity and cell adhesion strength using TEER (A) and Dispase (B) assays. N=3 for each; error bars represent s.e.m. (C) Schematic diagram of different types of cell junctions assayed by FRAP, showing junctions between Ecad-GFP Hi cells (Hi), between Ecad-GFP Lo cells (Lo), between Ecad-GFP Hi cells and the parental cell line (P) expressing endogenous E-cadherin, and between Ecad-GFP Hi cells and L cells (Ø) expressing no E-cadherin. (D) Fi of Ecad-GFP is the same in Ecad-GFP Hi and Ecad-GFP Lo cells (blue and green circles respectively), however T1/2 is shorter for Ecad-GFP Lo cells. The level of Ecad-GFP expression in the neighboring cell of a junction also affects E-cadherin dynamics. When FRAP is measured between cells expressing high levels of Ecad-GFP and no Ecad-GFP (black circles) both T1/2 and Fi are significantly reduced. Fi and T1/2 are further decreased in the absence of trans-dimer formation (red circle). Value for Ecad-GFP is included from Fig. 2C for comparison; see supplementary material Table S2 for list of all FRAP values. Error bars represent s.e.m. Zahra Erami et al. Biology Open 2015;bio.014159 © 2015. Published by The Company of Biologists Ltd