Distribution of deletions and insertions in the ade6-(GACC)7ΔT repeat. Distribution of deletions and insertions in the ade6-(GACC)7ΔT repeat. (A) Deletions and insertions within the (GACC)7ΔT repeat that lead to Ade+ can be distinguished by their color. ade6-(GACC)7ΔT mutants form red colonies on medium with limited amount of adenine due to a defective ade6 gene. They can revert to Ade+ by deletion of two or five repeat units, producing white Ade+, or by insertion of one or four repeat units, producing pink Ade+. (B) Percentage of deletions in the various strain backgrounds. Wild type, msh3, and exo1 mainly reverted to Ade+ by deletions, while msh2, msh6, and mlh1 mutants mainly reverted by insertions. Significantly different to wild type: ** p < 0.01; *** p < 0.001. Shown are average values with SDs. Reversion spectra are also presented as pie charts, with the proportion of deletions and insertions indicated in white and pink, respectively. Desirée Villahermosa et al. G3 2017;7:1463-1473 ©2017 by Genetics Society of America