Inhibition of PI3K or SERCA phenocopies polycystin loss of function.

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Inhibition of PI3K or SERCA phenocopies polycystin loss of function. Inhibition of PI3K or SERCA phenocopies polycystin loss of function. Control untreated (A) or DMSO-treated (B) embryos at 48 hpf show no col2a1 expression in the notochord. (C) Treatment with 50 μM cyclopiazonic acid, an inhibitor of the ER calcium uptake ATPase SERCA, results in elevated expression of col2a1 in the notochord. (D–F) The PI3K inhibitor wortmannin causes a dose-dependent increase in the expression of col2a1 in the notochord. (G–I) Similarly, the PI3K inhibitor LY294002 causes a dose-dependent increase in the expression of col2a1 in the notochord. Steve Mangos et al. Dis. Model. Mech. 2010;3:354-365 ©2010 by The Company of Biologists Limited