Erythroid differentiation of human CD34+ cells promoted by AVP

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Fig. 2. Erythroid differentiation of human CD34+ cells promoted by AVP. Erythroid differentiation of human CD34+ cells promoted by AVP. (A) Both AVP and AVPR1B agonist cause HSPCs (CD34+) to proliferate in vitro. This effect was blocked by a specific AVPR1B antagonist. Data and error bars are means + SEM; ns, not significant. *P < 0.05. OD 450, optical density at 450 nm. (B) Colony-forming assays were conducted to determine burst-forming unit–erythroid (BFU-E) and colony-forming unit–erythroid (CFU-E) colony numbers when CD34+ cells were exposed to AVP or a specific AVPR1B agonist added to the methylcellulose. AVP and the AVPR1B agonist significantly increased BFU-E colonies but not CFU-E. n = 3 independent donors and two replicates each. Data and error bars are means + SEM. *P < 0.05. (C) Colony-forming assays were conducted after 48 hours of treatment with AVP or AVPR1B agonist. Both AVP and AVPR1B agonist significantly increased CFU-E, but only the AVPR1B agonist increased BFU-E colonies. n = 2 independent donors and two replicates each. Data and error bars are means + SEM. *P < 0.05, ***P < 0.001. (D) May-Grünwald/Giemsa staining of cells from BFU-E colonies. Cells were harvested by Cytospin after growing for 10 days in control medium or medium with 10 nM AVP. Most of the cells in the control colonies are basophilic erythroblasts. Addition of AVP seems to accelerate differentiation; many reticulocytes can be seen, indicated by the arrowhead. The asterisk is placed between two erythrocytes that are in the process of enucleation to become reticulocytes. Scale bars, 100 μm (low magnification; left) and 10 μm (high magnification; right). (E) Human CD34+ cells were cultured with or without AVP. The cells were counted every 3 days. Each graph represents one donor. (F) Western blots of p-STAT5 and signal transducer and activator of transcription 5 (STAT5). Human CD34+ cells were challenged with erythropoietin (EPO), AVP, or EPO + AVP on days 9 and 12. On day 9, the effect of EPO + AVP on STAT5 phosphorylation was markedly greater than the effects of EPO or AVP alone. On day 12, the late-stage erythroid cells were more responsive to AVP than EPO, and the two agents no longer appeared to act in concert. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Balázs Mayer et al., Sci Transl Med 2017;9:eaao1632 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works