The Importance of Cationic Amino Acid Transporter Expression in Human Skin  Astrid Kehlen  Journal of Investigative Dermatology  Volume 121, Issue 6, Pages 1552-1553 (December 2003) DOI: 10.1046/j.1523-1747.2003.12646.x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Quantification of the basic expression of genes hCAT1, hCAT2b, and hCAT4 in NHEK, HaCaT, and HDMEC. Untreated NHEK, HaCaT, and HDMEC were harvested and used in a real-time PCR in cDNA-transcribed RNA. Each product could be amplified by using specific primers. Quantification was possible with the intercalating substance SybrGreen, which was run with every batch. Three independent experiments were performed for each. Journal of Investigative Dermatology 2003 121, 1552-1553DOI: (10.1046/j.1523-1747.2003.12646.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Quantification of expression of genes hCAT1, hCAT2b, and hCAT4 in NHEK and HDMEC after incubation with a cocktail of tumor necrosis factor α, interleukin-1β, and interferon-γ (1000 U per ml in NHEK or 800 U per ml in HDMEC). After incubation with 1000 U per ml tumor necrosis factor α, interleukin-1β, and interferon-γ NHEK, HDMEC, and HaCaT cells were harvested and used in a real-time PCR in cDNA-transcribed RNA. Each product could be amplified by using specific primers. Quantification was possible with the intercalating substance SybrGreen, which was run with every batch. Three independent experiments were performed for each. *Significant changes with p<0.05 versus untreated control. Journal of Investigative Dermatology 2003 121, 1552-1553DOI: (10.1046/j.1523-1747.2003.12646.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions