Fig. 4. Downregulation of KDM4C restores the integrity of DNA mismatch repair.(A) An outline describing the experimental flow to assess the effect of KDM4C.

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Fig. 4. Downregulation of KDM4C restores the integrity of DNA mismatch repair.(A) An outline describing the experimental flow to assess the effect of KDM4C downregulation on the rate of mutation frequency at the HPRT gene. Downregulation of KDM4C restores the integrity of DNA mismatch repair.(A) An outline describing the experimental flow to assess the effect of KDM4C downregulation on the rate of mutation frequency at the HPRT gene. Cells were cultured with doxycycline for 7 days to induce EGFP-KDM4C expression, then the doxycycline was removed to shutdown the expression of EGFP-KDM4C and subsequently the mutation frequency at the HPRT gene was determined. (B) Western blot showing that doxycycline removal suppresses the expression of EGFP-KDM4C fusion. Protein lysates from untreated and doxycycline-treated U2OS-TetON-EGFP-KDM4C cells were immunoblotted using the indicated antibodies. (C) A model showing that KDM4A-C overexpression diminishes H3K36me3 signal, impairs MSH6 foci and impairs the integrity of DNA MMR pathway. Samah W. Awwad, and Nabieh Ayoub Biology Open 2015;bio.201410991 © 2015. Published by The Company of Biologists Ltd