Differential Cre-ERT activity and PTEN deletion in endometrial epithelial and stromal compartments. Differential Cre-ERT activity and PTEN deletion in.

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Differential Cre-ERT activity and PTEN deletion in endometrial epithelial and stromal compartments. Differential Cre-ERT activity and PTEN deletion in endometrial epithelial and stromal compartments. (A) Schematic showing the experimental setup for tamoxifen (TAM) treatment. Briefly, mice were weaned 3 weeks after birth and genotyped. At 4–5 weeks old, animals were given a single intraperitoneal dose of tamoxifen, or corn oil as a vehicle (con). Mice were sacrificed 7 days later. (B) Tamoxifen induces a dose-dependent deletion of PTEN. PTEN immunohistochemistry corresponding to CAG-Cre-ERT+/−PTENfl/fl 7 days after tamoxifen injection. Note that stromal cells remained positive for PTEN staining. (C) Quantification of the percentage of endometrial epithelial cells displaying negative for PTEN immunostaining after injection of the indicated doses of tamoxifen. (D) Analysis of tamoxifen-induced recombination in CAG-Cre-ERT mT/mG reporter mice. Representative images corresponding to CAG-Cre-ERT+/−mT/mG+/− uterus 1 week after tamoxifen injection are shown. Note that, upon tamoxifen treatment, only epithelial cells showed a switch from red to green fluorescence. Cristina Mirantes et al. Dis. Model. Mech. 2013;6:710-720 © 2013. Published by The Company of Biologists Ltd