Osteogenesis in monolayer HLA-B27 transduced hPDCs. Osteogenesis in monolayer HLA-B27 transduced hPDCs. (A) Expression of OCN, ALP and RUNX2 measured by quantitative RT-PCR in the HLA-B27 and HLA-B7 hPDCs. Statistical analysis was performed by two-way ANOVA. p<0.05 was considered as statistically significant. (B) ALP activity assay. Data points are shown as average and SEM (n=3). (C) Alizarin red staining at the different time points. ALP, alkaline phosphatase; OCN, osteocalcin. Barbara Neerinckx et al. RMD Open 2017;3:e000451 Copyright © BMJ Publishing Group & EULAR. All rights reserved.