Analysis of plasmablast responses in one naïve and three DENV-experienced donors. Analysis of plasmablast responses in one naïve and three DENV-experienced.

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Analysis of plasmablast responses in one naïve and three DENV-experienced donors. Analysis of plasmablast responses in one naïve and three DENV-experienced donors. (A) Flow cytometric analysis of plasmablast responses in one naïve and three DENV-experienced donors during ongoing ZIKV infection. Plasmablasts are defined herein as CD19+CD3−CD20−/loCD38hiCD27hi cells. Plots shown are gated for CD3−CD20−/lo cells, with the CD27hiCD38hi plasmablasts marked by a box. The frequency of plasmablasts within the CD3−CD20−/lo population is shown next to the gate. The numbers in parentheses show the percentage of peripheral B cells that are plasmablasts. Flow cytometry data were analyzed using FlowJo software (version 10.0.8). (B) Load of somatic mutations [expressed as the number of nucleotide (NT) substitutions] in VH and VL in plasmablast-derived mAbs isolated from a DENV-naïve (donor 1) and three DENV-experienced donors (donors 2 to 4). All VH and VL chains are shown, regardless of whether the paired antibody chain was recovered. (C) Clonal lineage analysis. Heavy-chain sequences were assigned to lineages using Clonify (52). Each lineage is represented as a segment proportional to the lineage size. The total number of recovered heavy chains is shown in the center of each pie. All lineages that contain only a single sequence are combined and shown as a single gray segment. Thomas F. Rogers et al. Sci. Immunol. 2017;2:eaan6809 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works