Fig. 2. Proportion of motile objects and track length

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Fig. 2. Proportion of motile objects and track length Fig. 2. Proportion of motile objects and track length.(A) Proportion of moving objects as a percentage of the total puncta detected. Proportion of motile objects and track length.(A) Proportion of moving objects as a percentage of the total puncta detected. Statistical significance was tested using an ANOVA with a Dunnett's post-hoc test. (B) Quantification of time-lapse imaging. Images were processed to generate maximum intensity projections as shown in Fig. 1. Track lengths were then automatically measured in Volocity and plotted. Each spot represents an individual particle. Measurements are derived from 3 independent experiments, 3–8 cells in each experiment, total of 75 objects. Statistical significance was tested using an ANOVA with a Dunnett's post-hoc test. Red asterisks indicate statistically detectable decrease in track length; green asterisks represent a statistically detectable increase in track length. Anna K. Brown et al. Biology Open 2014;bio.20147633 © 2014. Published by The Company of Biologists Ltd