Effect of IL-13 receptor α2 levels on the biological activity of IL-13 variant R110Q Allison-Lynn Andrews, PhD, Fabio Bucchieri, MD, Kazuhiko Arima, MD,

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Presentation transcript:

Effect of IL-13 receptor α2 levels on the biological activity of IL-13 variant R110Q Allison-Lynn Andrews, PhD, Fabio Bucchieri, MD, Kazuhiko Arima, MD, Kenji Izuhara, MD, Stephen T. Holgate, DSc, Donna E. Davies, PhD, John W. Holloway, PhD Journal of Allergy and Clinical Immunology Volume 120, Issue 1, Pages 91-97 (July 2007) DOI: 10.1016/j.jaci.2007.04.026 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Kinetic analysis of R110Q by SPR. Representative sensograms of R110Q (solid line; lower to upper curve: 20, 40, 60, 80 and 100 nmol/) and wild type IL-13 (dashed line) binding to the surface of CM5 sensor chip coated with shIL-13Rα2. A slower association rate was observed for the binding of R110Q to shIL-13Rα2 compared with wild-type IL-13. There was no significant difference in the rate of dissociation. Data are means ± SDs (n = 5). Journal of Allergy and Clinical Immunology 2007 120, 91-97DOI: (10.1016/j.jaci.2007.04.026) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Functional consequences of R110Q expression. Primary human fibroblasts were grown to confluence before being treated with either IL-13 (10 ng/mL) or R110Q (10 ng/mL) for 24 hours. Supernatants were assayed for eotaxin by ELISA. IL-13Rα2 levels were determined by fluorescence-activated cell sorting. Fibroblasts with low levels of IL-13Rα2 showed a significant difference in eotaxin release between cells treated with IL-13 and R110Q. Symbols represent individual volunteers (n = 13). Statistical analysis was performed by using the Wilcoxon rank-sum test. Journal of Allergy and Clinical Immunology 2007 120, 91-97DOI: (10.1016/j.jaci.2007.04.026) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Addition of neutralizing IL-13Rα2 antibody on eotaxin release. Primary human fibroblasts were grown to confluence before being treated with either IL-13 (10 ng/mL) or R110Q (10 ng/mL) for 24 hours in the presence or absence of a neutralizing IL-13Rα2 antibody. Significant difference in eotaxin release between those cells treated with IL-13 and R110Q was abolished by the addition of the neutralizing antibody. Symbols represent individual volunteers (n = 13). Statistical analysis was performed by using the Wilcoxon rank-sum test. Journal of Allergy and Clinical Immunology 2007 120, 91-97DOI: (10.1016/j.jaci.2007.04.026) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 IL-13 and R110Q induced STAT6 phosphorylation. A, Primary human fibroblasts were treated with serum free medium (SFM), IL-13, or R110Q as indicated. Cells were then solubilized in sample buffer before Western blotting and probing with a phospho-STAT6 antibody. B, STAT6 phosphorylation was quantified by densitometry and normalized to STAT6 loading. Data are presented as means ± SDs (n = 3). IL-13. R110Q. ∗P < .05. Journal of Allergy and Clinical Immunology 2007 120, 91-97DOI: (10.1016/j.jaci.2007.04.026) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Surface levels of IL-13Rα2 are an important regulatory factor. A, Fibroblasts with either low (1) or high (2) surface levels of IL-13Rα2 were treated with IL-13 or R110Q as indicated in the presence or absence of a neutralizing IL-13Rα2 antibody. B, The presence of the neutralizing antibody completely abolished the difference in STAT6 phosphorylation observed in fibroblasts with low surface levels of IL-13Rα2. Data are representative of 3 individual experiments. pSTAT6, PhosphoSTAT6. Journal of Allergy and Clinical Immunology 2007 120, 91-97DOI: (10.1016/j.jaci.2007.04.026) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions