Case report: Streptomycin-induced anaphylactic shock during oocyte retrieval procedures for in vitro fertilization Motoyasu Iikura, MD, PhDa, Masao Yamaguchi,

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Case report: Streptomycin-induced anaphylactic shock during oocyte retrieval procedures for in vitro fertilization Motoyasu Iikura, MD, PhDa, Masao Yamaguchi, MD, PhDa, Koichi Hirai, MD, PhDb, Akihiko Suenaga, MD, PhDc, Toshihiro Fujiwara, MD, PhDc, Tomoyuki Fujii, MD, PhDc, Yuji Taketani, MD, PhDc, Kazuhiko Yamamoto, MD, PhDa Journal of Allergy and Clinical Immunology Volume 109, Issue 3, Pages 571-572 (March 2002) DOI: 10.1067/mai.2002.121533 Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 1 Results of histamine-release testing of basophils from patient 1 on day 0 and after 3-day culture with IL-3 but without any antibiotics (A ), cells from patient 2 on day 0 (B ), and cells from a normal subject sensitized with serum from patient 1 and patient 2 (C ). Data are mean values of duplicate determinations. aIgE , Anti-IgE; Ca , calcium ionophore A23187. C , Basophils (from a donor of the same ABO blood group as patient 1 or 2) were treated at 4°C for 5 minutes with acetate buffer (pH 3.6) to elute surface-bound IgE, sensitized at 37°C for 2 hours with serum obtained from patient 1 or 2, and then stimulated with SM. Control unsensitized cells or cells sensitized with heated (at 56°C for 2 hours) serum showed no release in response to SM challenge (data not shown). Data are representative of 2 experiments showing similar results. Journal of Allergy and Clinical Immunology 2002 109, 571-572DOI: (10.1067/mai.2002.121533) Copyright © 2002 Mosby, Inc. Terms and Conditions