Localisation of epithelial markers in mMEC cultures.

Slides:

Advertisements

Similar presentations

Mitochondria localize to both the front and the rear of neutrophils.

Advertisements

Supplementary figure 1 D

Primary culture of mouse middle ear epithelial cells.

The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles. The neuroepithelium of mid-neurulation mammalian embryos.

Fig. 2 In vitro assessment of hESC-RPE cell sheets.

Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+ cells Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+

Effectively regenerating livers transiently accumulate proliferative IGF2BP3-positive cells. Effectively regenerating livers transiently accumulate proliferative.

Fig. 6 Photoreceptor cell survival in the RCS rat retina after transplantation with hESC-RPE cell sheets. Photoreceptor cell survival in the RCS rat retina.

Inducible DM1 model displays increased autophagy.

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Nephrogenesis in vitro can be rescued in 3D co-cultures by siRNA treatment of Renca cells. Nephrogenesis in vitro can be rescued in 3D co-cultures by siRNA.

Fig. 5. Differentiated cells sort to the outer layer, regardless of E- or N-cadherin status.Wildtype and mutant ES cells were distinguished by GFP expression.

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

Notch pathway components in serial sections of the human colon.

A subset of Trp53CKO fiber cells fail to exit the cell cycle, but express fiber-cell-specific markers. A subset of Trp53CKO fiber cells fail to exit the.

Time course of PKA subunit expression using CMV or foxj1 promoter in human airway epithelial cells. Time course of PKA subunit expression using CMV or.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Indirect fluorescence assay of N. gruberi cells.

Fig. 1. Morphological and growth characterization of hBMCs and hPDCs

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Challenge with oxazolone results in epithelial hyperplasia and influx of inflammatory cells into the epidermis and dermis and keratosis as observed in.

Fig. 1. Ovarian cancer spheroids can bud from a monolayer

Apical localization of PKA fusion constructs in infected and fully differentiated human airway epithelial cells. Apical localization of PKA fusion constructs.

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

Increased osteoclastogenesis in the mandibular bone of Fgfr2+/S252W embryos at E16.5. Increased osteoclastogenesis in the mandibular bone of Fgfr2+/S252W.

Morphological defects of hi559 GI tract.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen.

Overexpression of individual signaling pathway components does not phenocopy the cell-spreading phenotype induced by elevated Hipk. Overexpression of individual.

MiR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion. miR-200c/141 overexpression in LS-8 cells induces E-cad expression.

The mutants exhibit congenital hypothyroidism.

Fig. 6. Apical polarity of primitive endoderm cells on surface of embryoid bodies.Embryoid bodies were analyzed by immunofluorescence microscopy for GATA4.

Rgs16::GFP expression in pancreatic neoplasia.

Homozygous col22a1−/− embryos show increased percentage of hemorrhages when raised at increased temperature. Homozygous col22a1−/− embryos show increased.

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

Overexpression of Lef-1 partially rescues tooth arrest in Sox2cKO embryos. Overexpression of Lef-1 partially rescues tooth arrest in Sox2cKO embryos. (A,B)

Fig. 1. Phenotypic characterisation of primary human tubular epithelial cells and human renal fibroblasts. Phenotypic characterisation of primary human.

Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. Transbilayer distribution of cholesterol in.

EpiDEG efficiently degrades GFP-tagged proteins that localize to different subcellular localizations. epiDEG efficiently degrades GFP-tagged proteins that.

Sox2 regulates dental epithelial stem cell proliferation and differentiation. Sox2 regulates dental epithelial stem cell proliferation and differentiation.

Effect of Dipy on the expression of chondrogenic markers.

Nerve conduction slowing and DRG sensory neuron and nuclear atrophy in 16-week-old diabetic mice. Nerve conduction slowing and DRG sensory neuron and nuclear.

Z-stack compressions showing triple labelling of different cell types in the gills of bowfin with antibodies for serotonin (5-HT, green) and HNK-1 (magenta),

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

Organoid-derived transplantable corneal epithelial grafts on human amniotic membrane substrate. Organoid-derived transplantable corneal epithelial grafts.

IGF1R is present in endometrial luminal epithelium at the receptive phase. IGF1R is present in endometrial luminal epithelium at the receptive phase. Endometrial.

c-Met overexpression promotes cell rounding.

ALS-CSF induces atrophy of type 2 fibres in EDL muscle of neonatal rats. ALS-CSF induces atrophy of type 2 fibres in EDL muscle of neonatal rats. (A,B)

ER stress and ultrastructural pathology of IECs

Peroxisome speeds were slower in patient and control cells.

Fig. 1. Cell adhesion molecule expression and the aggregation of wildtype and mutant ES cells.(A) Wildtype (WT), E-cadherin null (9J), and N-cadherin null.

Proliferation on soft matrices is decoupled from myosin light chain phosphorylation, cell tractions, and focal adhesions. Proliferation on soft matrices.

Immunofluorescence staining of Gli in the osmoregulatory tissues of Aedes aegypti larva. Immunofluorescence staining of Gli in the osmoregulatory tissues.

Histone crotonylation in kidney tubular cells.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Proliferative and morphological defects following TbCALM and TbEpsinR depletion. Proliferative and morphological defects following TbCALM and TbEpsinR.

Effects of paclitaxel and vinblastine.

Lack of Ctip2 is associated with impaired proliferation and delayed onset of differentiation during early stages of epidermal development. Lack of Ctip2.

BMOL3 LPCs can differentiate towards the hepatocyte lineage.

Ciliated cell expression of PKA fusion constructs in infected and fully differentiated human airway epithelial cells. Ciliated cell expression of PKA fusion.

Disruption of the tubule formation by immortalized cells during nephrogenesis in vitro. Disruption of the tubule formation by immortalized cells during.

Subnuclear localization of HA-tagged EBNA1 protein.

2DG suppresses of lamellipodia and filopodia and causes disorganization of F-actin filaments in murine endothelial cells. 2DG suppresses of lamellipodia.

Co-expression of periostin and Sox10 in DRG and DRG explant cultures.

Ca2+-mediated differentiation of primary mouse keratinocytes is independent of epiplakin. Ca2+-mediated differentiation of primary mouse keratinocytes.

Volume 16, Issue 2, Pages (February 2015)

Presentation transcript:

Localisation of epithelial markers in mMEC cultures. Localisation of epithelial markers in mMEC cultures. (A,C) Immunofluorescence confocal images (representative of three independent batches) showing abundant expression of the basal cell marker, P63; limited expression of the secretory protein, Bpifa1 (A), and no expression of goblet cell marker, Muc5B and the ciliated marker, FoxJ1 (C) in undifferentiated ALI day 0 mMEC cultures. (B,D,E,F) Differentiated mMEC ALI day 14 cultures showing expression of secretory cells positive for Bpifa1 (B), lactotransferrin (E), Reg3γ (F), goblet cells positive for Muc5B and ciliated cells positive for FoxJ1 (D). Cross-sections through both axes of the membrane are shown beneath and to the right beyond the white lines. (G,H) High magnification z-stack cross sections of nuclei stained with DAPI shows that ALI day 0 cells form a flat monolayer (G) whereas ALI day 14 cells are a combination of pseudostratified elevated cells (arrow) and flatter cells (asterisk) (H). Scale bars: 50 μm. Apoorva Mulay et al. Dis. Model. Mech. 2016;9:1405-1417 © 2016. Published by The Company of Biologists Ltd