Volume 62, Issue 4, Pages (October 2002)

Slides:

Advertisements

Similar presentations

Volume 70, Issue 7, Pages (October 2006)

Advertisements

Volume 59, Issue 5, Pages (May 2001)

Volume 65, Issue 6, Pages (June 2004)

l-Carnitine is an osmotic agent suitable for peritoneal dialysis

Volume 56, Issue 4, Pages (October 1999)

Beta blockers in the management of chronic kidney disease

l-Carnitine is an osmotic agent suitable for peritoneal dialysis

Effects of inflammatory cytokines on the release and cleavage of the endothelial cell–derived ultralarge von Willebrand factor multimers under flow by.

Anemia management in chronic kidney disease

Exposure to fluid shear stress modulates the ability of endothelial cells to recruit neutrophils in response to tumor necrosis factor-α: a basis for local.

Volume 60, Issue 2, Pages (August 2001)

Adrian Schreiber, Friedrich C. Luft, Ralph Kettritz

Volume 70, Issue 7, Pages (October 2006)

l-Carnitine is an osmotic agent suitable for peritoneal dialysis

Lorraine Harper, Paul Cockwell, Dwoma Adu, Caroline O.S. Savage

C-reactive protein and dialysis access

Volume 62, Issue 1, Pages (July 2002)

Differential effects of 19-nor-1,25-(OH)2D2 and 1α-hydroxyvitamin D2 on calcium and phosphorus in normal and uremic rats Eduardo Slatopolsky, Mario Cozzolino,

Neal X. Chen, Kalisha D. O'Neill, Danxia Duan, Sharon M. Moe

Volume 85, Issue 3, Pages (March 2014)

Volume 60, Issue 4, Pages (October 2001)

Effect of retroviral transduction on human endothelial cell phenotype and adhesion to Dacron vascular grafts Ronald J. Jankowski, MS, Donald A. Severyn,

Lysophosphatidic acid-induced proliferation in opossum kidney proximal tubular cells: Role of PI 3-kinase and ERK Richard J. Dixon, Nigel J. Brunskill

Hsueh Yang, Gabrielle Curinga, Cecilia M. Giachelli

Advanced glycation endproducts induce podocyte apoptosis by activation of the FOXO4 transcription factor P.Y. Chuang, Q. Yu, W. Fang, J. Uribarri, J.C.

A new era in phosphate binder therapy: What are the options?

The importance of increased dialysis and anemia management for infant survival in pregnant women on hemodialysis Sai Subhodhini Reddy, Jean L. Holley

Volume 67, Issue 3, Pages (March 2005)

Joseph W. Eschbach, John W. Adamson Kidney International

Jeffrey M. Rhodes, MD, Anna F. Tilberg, MS, Robert R.M. Gifford, MD

Volume 86, Issue 2, Pages (August 2014)

Volume 70, Issue 6, Pages (September 2006)

Volume 62, Issue 1, Pages (July 2002)

Volume 60, Issue 3, Pages (September 2001)

Bisphosphonate safety and efficacy in chronic kidney disease

Volume 65, Issue 2, Pages (February 2004)

Izabella Z.A. Pawluczyk, Kevin P.G. Harris Kidney International

Volume 62, Issue 4, Pages (October 2002)

Relationship between methylmalonic acid and cobalamin in uremia

Blood pressure targets in hemodialysis patients

Volume 65, Issue 6, Pages (June 2004)

Volume 69, Issue 1, Pages (January 2006)

John H. Wiessner, Linda Y. Hung, Neil S. Mandel Kidney International

Volume 60, Issue 5, Pages (November 2001)

Volume 69, Issue 12, Pages (June 2006)

Volume 69, Issue 3, Pages (February 2006)

Volume 62, Issue 4, Pages (October 2002)

Kidney failure and the gut: p-cresol and the dangers from within

Alternate-day dialysis may be needed for hemodialysis patients

Volume 59, Issue 2, Pages (February 2001)

Inflammatory signals associated with hemodialysis

Michael W. Steffes, Derek Schmidt, Rebecca Mccrery, John M. Basgen

Volume 55, Issue 6, Pages (June 1999)

Lu-Cheng Cao, Thomas Honeyman, Julie Jonassen, Cheryl Scheid

Fibrinolytic activity of human mesothelial cells is counteracted by rapid uptake of tissue- type plasminogen activator Thomas Sitter, Karin Toet, Paul.

Volume 54, Issue 5, Pages (November 1998)

Steven J. Harper, Charles R.V. Tomson, David O. Bates

Wei-Teing Chen, Horng-Chin Yan, Fu-Chiu Yu Kidney International

Current status of maintenance hemodialysis in Beijing, China

Uremic autonomic neuropathy studied by spectral analysis of heart rate

Hemodialysis and L-arginine, but not D-arginine, correct renal failure-associated endothelial dysfunction Malcolm F. Hand, William G. Haynes, David J.

Volume 80, Issue 10, Pages (November 2011)

Volume 64, Issue 5, Pages (November 2003)

Volume 70, Issue 7, Pages (October 2006)

Volume 71, Issue 9, Pages (May 2007)

Volume 70, Issue 5, Pages (September 2006)

Volume 54, Issue 4, Pages (October 1998)

Rebound kinetics of β2-microglobulin after hemodialysis

A. Khwaja, C.C. Sharpe, M. Noor, B.M. Hendry Kidney International

Stephen Pastan, J. Michael Soucie, William M. McClellan

Presentation transcript:

Volume 62, Issue 4, Pages 1358-1363 (October 2002) Enhanced adherence of human uremic erythrocytes to vascular endothelium: Role of phosphatidylserine exposure Mario Bonomini, Vittorio Sirolli, Federico Gizzi, Silvio Di Stante, Alfredo Grilli, Mario Felaco Kidney International Volume 62, Issue 4, Pages 1358-1363 (October 2002) DOI: 10.1111/j.1523-1755.2002.kid560.x Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 1 Adherence of erythrocytes from uremic patients on hemodialysis (N = 20) and normal subjects (N = 20) to endothelial cell monolayers. Adherence was by a static assay. Error bars depict the standard error. *Significant difference between normal and uremic erythrocytes adherent to HUVEC. Kidney International 2002 62, 1358-1363DOI: (10.1111/j.1523-1755.2002.kid560.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 2 Correlation in chronic uremic patients on hemodialysis therapy (N = 20) between phosphatidylserine (PS)-exposing normal human erythrocyte (RBC) and static RBC adherence to endothelial cell monolayers. The FITC-annexin V positive RBC population was measured by FACS analysis (r = 0.88; P < 0.0001). Kidney International 2002 62, 1358-1363DOI: (10.1111/j.1523-1755.2002.kid560.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 3 Effect of PS competitors on uremic erythrocyte adherence to endothelial cell monolayers. Erythrocyte suspensions from hemodialysis patients (N = 10) were incubated for five minutes with one of the following before the static adherence assay with HUVEC: buffer (Control), annexin V, PS vesicles (30% PS, 70% PC, 5mmol/L lipid), PC vesicles (100% PC, 5mmol/L lipid). Error bars depict the standard error. *Significantly different from the control. Kidney International 2002 62, 1358-1363DOI: (10.1111/j.1523-1755.2002.kid560.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 4 Uremic (□) and normal (▪) RBC adherence to immobilized proteins. Multiwell slides were coated with 50 μg/mL of indicated proteins and then blocked with 1% BSA for 60 minutes. A washed suspension of RBC (1% Hmt in HBSS) was placed on the immobilized proteins for 45 minutes, followed by three washes with PBS. *Significant difference between normal and uremic RBC adherence to TSP (P < 0.001, N = 10). Kidney International 2002 62, 1358-1363DOI: (10.1111/j.1523-1755.2002.kid560.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 5 Effect of monoclonal antibody to thrombospondin (TSP) on uremic RBC adherence to endothelial cell monolayers. HUVEC monolayers were incubated with monoclonal antibody to TSP or a control monoclonal antibody (trpE) before being layered in a static assay with uremic RBC suspensions. Results are expressed as percentage of the buffer-treated control adherence, which is set to 100%. Error bars represent SEM calculated from the absolute values from 8 experiments and normalized relative to the adherence of the buffer-treated control. *Significant differences from the control. Kidney International 2002 62, 1358-1363DOI: (10.1111/j.1523-1755.2002.kid560.x) Copyright © 2002 International Society of Nephrology Terms and Conditions