Volume 59, Issue 3, Pages 1134-1141 (March 2001) Increased vascular growth in hemodialysis patients induced by platelet-derived diadenosine polyphosphates  Joachim Jankowski, Joost Hagemann, Min-Sung Yoon, Markus Van Der Giet, Nina Stephan, Walter Zidek, Hartmut Schlüter, Martin Tepel  Kidney International  Volume 59, Issue 3, Pages 1134-1141 (March 2001) DOI: 10.1046/j.1523-1755.2001.0590031134.x Copyright © 2001 International Society of Nephrology Terms and Conditions

Figure 1 [3H]-thymidine incorporation of vascular smooth muscle cells (VSMCs) stimulated by the supernatant of 106-stimulated platelets from hemodialysis patients and healthy control subjects in the absence (□) and presence (▪) of the selective P2-receptor blocker, PPADS. Ordinate: in counts/supernatant of 106-stimulated platelets. Data are means ± SEM (P < 0.05 compared with control). Kidney International 2001 59, 1134-1141DOI: (10.1046/j.1523-1755.2001.0590031134.x) Copyright © 2001 International Society of Nephrology Terms and Conditions

Figure 2 Reversed-phase chromatography of supernatant of platelets activated by thrombin from hemodialysis patients (A) and healthy control subjects (B) on an analytical reversed-phase, high-pressure liquid chromatography (HPLC) column. Kidney International 2001 59, 1134-1141DOI: (10.1046/j.1523-1755.2001.0590031134.x) Copyright © 2001 International Society of Nephrology Terms and Conditions

Figure 3 Characteristic positive-ion post-source decay (PSD) matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) mass spectrum of a fraction of reversed-phase chromatography. The underlying substance was identified as Ap4A. Abbreviations are: A′, adenine; A, adenosine; M, protonated parent ion; p, phosphate group (for example, Ap2 adenosine diphosphate). Abscissa is the relative mass/charge, m/z, z = 1; the ordinate denotes relative intensity in arbitrary units. Kidney International 2001 59, 1134-1141DOI: (10.1046/j.1523-1755.2001.0590031134.x) Copyright © 2001 International Society of Nephrology Terms and Conditions

Figure 4 Reversed-phase chromatography of homogenate of human platelets after affinity- and reversed-phase chromatography (conditions as described in Figure 2). Ap8A was added to the sample as an internal standard. Kidney International 2001 59, 1134-1141DOI: (10.1046/j.1523-1755.2001.0590031134.x) Copyright © 2001 International Society of Nephrology Terms and Conditions

Figure 5 Intracellular concentrations of the Ap3A, Ap4A, Ap5A, and Ap6A in platelets from hemodialysis patients. Data are means ± SEM (P < 0.01 compared with control). Kidney International 2001 59, 1134-1141DOI: (10.1046/j.1523-1755.2001.0590031134.x) Copyright © 2001 International Society of Nephrology Terms and Conditions