Overexpression of cx43 rescues smc3-dependent skeletal phenotypes

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Fig. 5. Overexpression of cx43 rescues smc3-dependent skeletal phenotypes. Overexpression of cx43 rescues smc3-dependent skeletal phenotypes. (A) Experimental timeline providing details of the fin amputation, MO injection/electroporation, heat shock and data analysis process. Fin amputation (50% level) was performed on transgenic hsp70:miR-133sppd48 fish (Tg+) and their siblings (Tg−). At 3 dpa, smc3 MO was injected in one half of the fin keeping the other half uninjected. This step was immediately followed by electroporating both sides of the fin. After a period of 4 h, the heat shock process began. At this point there were three groups of fish: (1) Tg+HS+, the transgenic-positive fish that were heat shocked at 37°C for 1 h; (2) Tg+HS−, the transgenic-positive fish but were not heat shocked; (3) Tg−HS+, the siblings (transgenic-negative) that were similarly heat shocked as Tg+HS+. At 4 dpa or 1 dpe, the Tg+HS+ fins were screened for positive GFP expression, which indicated transgene induction. The control groups (Tg+HS− and Tg−HS+) were GFP negative, indicating absence of transgene induction. For regenerate length and segment length measurement and data analysis, fins were calcein stained at 7 dpa or 4 dpe. (B) The graph reveals significant rescue of smc3-dependent regenerate and segment length defects in Tg+HS− smc3 knockdown fins compared to the control groups (Tg−HS+ and Tg+HS−). For each experiment n=8 fins per trial and three independent trials were performed. *P<0.05, two tailed unpaired Student's t-test. Data are mean±s.e.m. (C) Smc3 protein expression is nearly similar (90%) in the Tg-HS+ (lane 1) and Tg+HS+ (lane 2) fin lysates (normalized to Tubulin). In contrast, Cx43 protein is increased) in Tg+HS+ (lane 2) fin lysate compared to Tg−HS+ (lane 1) fin lysate, as expected. Similarly, GFP protein expression is also increased in Tg+HS+ fin lysate (lane 2) compared to Tg−HS+ fin lysate. Tubulin (50 kDa) was used as the loading control for all blots. ImageJ software was used for analysis of relative band intensity. Rajeswari Banerji et al. Biology Open 2017;6:1802-1813 © 2017. Published by The Company of Biologists Ltd