Calibration of cAMP and forskolin induced FRET ratio and CBF

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Calibration of cAMP and forskolin induced FRET ratio and CBF Calibration of cAMP and forskolin induced FRET ratio and CBF. Basolaterally permeabilized cells or non-permeabilized cells were perfused apically with forskolin, or basolaterally with cAMP. Calibration of cAMP and forskolin induced FRET ratio and CBF. Basolaterally permeabilized cells or non-permeabilized cells were perfused apically with forskolin, or basolaterally with cAMP. (A) Increases in FRET ratio (in AUs), (B) increases in CBF (% above baseline), (C) rate of the FRET ratio increases and (D) rate of CBF changes are shown for all stimulations. All results stem from at least eight cells from two different lungs in two different experiments (see text for more details). Values are plotted as the mean ± s.e.m. □, 20 μM cAMP; ♦, 50 μM cAMP; ○, 100 μM cAMP; ▪, 1 μM forskolin; ♦, 10 μM forskolin; •, 20 μM forskolin. See text for significance levels. Andreas Schmid et al. J Cell Sci 2006;119:4176-4186 © The Company of Biologists Limited 2006