Treatment of oocytes with LMB or mutation of the leucine-rich NES of Xp54 has little effect on nuclear export of mRNP particles. Treatment of oocytes with.

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Treatment of oocytes with LMB or mutation of the leucine-rich NES of Xp54 has little effect on nuclear export of mRNP particles. Treatment of oocytes with LMB or mutation of the leucine-rich NES of Xp54 has little effect on nuclear export of mRNP particles. (A) Stage III/IV oocytes expressing T7-p54 were injected with 0.1 μCi of 32P-CTP, and poly(A)+ RNP was extracted to assay radioactivity incorporated (columns) and T7-p54 present (immunoblot) in nuclei and cytoplasms after 16 and 28 hours. (B) Immunoprecipitation, with anti-T7 IgG, of poly(A)+ and poly(A)– RNP extracted from cytoplasms of stage III/IV oocytes expressing T7-p54 and mutant T7-p54NES– for 60 hours. In addition, poly(A)+ tails from cytoplasms of 50 oocytes expressing T7-p54NES– were run on glycerol gradients, and sedimentation fractions were analysed by immunoblotting for the T7-tag. The sedimentation of T7-p54NES– from 50 nuclei is also shown. David A. Smillie, and John Sommerville J Cell Sci 2002;115:395-407 © The Company of Biologists Limited 2002