GeneSwitch GAL4 lines carry drivers for GSC maintenance and differentiation niches. GeneSwitch GAL4 lines carry drivers for GSC maintenance and differentiation niches. Five-day (D)-old germaria are shown from GeneSwitch GAL4 (A) #1774>gfp, (B) #2261>gfp, (C) #2112>gfp, (D) #2305>gfp, (E) #2126>gfp and (F) #2312>gfp flies that were fed with RU486 (10 µg/ml) for two days. Germaria were stained with 1B1 (red, fusomes), LamC (red, terminal filament [TF] and cap cell nuclear envelope), and GFP (gray). (A’-F’) only show the GFP channel. White dashed lines encircle GSCs; yellow lines indicate the junction between GSCs and cap cells, and brackets indicate TFs. Scale bar is 10 µm. Yi-Teng Ke, and Hwei-Jan Hsu G3 2019;9:2007-2016 ©2019 by Genetics Society of America