Mammary cell proliferation is inhibited when LAD1 is depleted.

Slides:

Advertisements

Similar presentations

High-dimensional analysis of lymphoid CD4+ T cells identified distinct TFH cell subsets in HIV+ patients and HCs. High-dimensional analysis of lymphoid.

Advertisements

Identification of combination treatment–responsive dysfunctional tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

Immunologic responses after the MN-mediated cancer immunotherapy.

Cytotoxicity of MSP samples to normal and cancer cell lines.

Nuclear calcium signaling drives nuclear actin polymerization in T cells. Nuclear calcium signaling drives nuclear actin polymerization in T cells. (A)

TPAD controller performance for three force components.

Antitumor effect of local cancer immunotherapy treatment toward distant B16F10 tumors. Antitumor effect of local cancer immunotherapy treatment toward.

TPAD training protocol.

Tfr cells’ transcriptomic profile distinguishes them from Treg and Tfh cells. Tfr cells’ transcriptomic profile distinguishes them from Treg and Tfh cells.

Examples of AEGIS autonomous target selection.

Human PBMC-derived MERS-CoV–specific T cells are multifunctional.

Group data during free walking between sessions 1 and 16.

Virus-specific T cell responses are detected in all MERS survivors.

Deficiency in myeloid-resident NRP1 affects systemic metabolism.

Platelets are required for hFcγRIIA-induced anaphylaxis.

NRP1-expressing myeloid cells influence adipocyte hypertrophy, development of fatty liver, and CLSs. NRP1-expressing myeloid cells influence adipocyte.

Human cells produce type I and III IFNs upon Af stimulation.

TRAF4 is required for EGFR activation in response to EGF stimulation.

Tukey boxplots overlaid on data points from objective and subjective measures, displaying results from study 1. Tukey boxplots overlaid on data points.

Characterization of the light-responsive transdermal MNs.

Donor and recipient BAL T cells are phenotypically and functionally memory T cells. Donor and recipient BAL T cells are phenotypically and functionally.

Blood Tfr cells show expression of follicular and regulatory markers.

Fig. 6 In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss. In utero injection of inflammatory.

Dectin-1 is repressed in allergic individuals.

Low accessibility to Ag for CTLs leads to low proliferation of effectors. Low accessibility to Ag for CTLs leads to low proliferation of effectors. Intravital.

AMPK directly increases mTORC2 catalytic activity.

LIMK1 inhibition protects against Aβ-induced neuronal hyperexcitability. LIMK1 inhibition protects against Aβ-induced neuronal hyperexcitability. (A) Representative.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

Microrobots with different cell-carrying capacities under different grid lengths (lg) and burr lengths (lb). Microrobots with different cell-carrying capacities.

Fig. 4 Rotation of nuclei in oocytes in the primordial follicles.

Global analysis of RV[S/T]F motif phosphorylation during mitosis.

Effects of NAT10 inhibition or depletion on gene expression.

Slc7a5 deficiency stimulates osteoclastogenesis in vitro.

Blood Tfr cells do not show specialized humoral regulatory capacity.

CD25 expression identifies two transcriptionally distinct subsets of very early effector cells. CD25 expression identifies two transcriptionally distinct.

Memory-biased TCRs induce weaker TCR signals than effector-biased TCRs in vitro. Memory-biased TCRs induce weaker TCR signals than effector-biased TCRs.

Fluorescence response of actuator.

BMS blocks functional responses in primary immune cells driven by IFNα

Fig. 6 RUNX/CBFB interaction inhibitor, Ro5-3335, significantly decreases mouse neurofibroma growth in vivo. RUNX/CBFB interaction inhibitor, Ro5-3335,

Fig. 2 ODN enhances EV transfer between cells expressing TLR9.

S-Affs colocalize with SUMO in mammalian cells.

by Emilie Clement, Hiroyuki Inuzuka, Naoe T

Fig. 6 WPS imaging of different chemical components in living cells.

Fig. 4 Cdc42 enhances the cellular uptake of EVs.

AEGIS autonomous targeting process.

Examples of AEGIS autonomous target selection.

Fig. 3 Hydrostatic pressure induces intracellular calcium increase via TRPM7 activation. Hydrostatic pressure induces intracellular calcium increase via.

Fig. 4. Paclitaxel promotes the expression of invasive isoforms of MENA in the primary breast cancer microenvironment. Paclitaxel promotes the expression.

by Geoffrey A. Smith, Jack Taunton, and Arthur Weiss

Candesartan diminishes SynO-mediated morphofunctional responses in microglia. Candesartan diminishes SynO-mediated morphofunctional responses in microglia.

Fig. 5 Increased myometrial cell contractility in response to fetal T cells from preterm infants. Increased myometrial cell contractility in response to.

IFN treatment of human midgestation villous explants induces syncytial knot formation. IFN treatment of human midgestation villous explants induces syncytial.

Human basophils are unresponsive to contact-dependent or contact-independent inhibition by Tregs. Human basophils are unresponsive to contact-dependent.

Snail-induced multinucleation follows midbody persistence.

Fig. 2 BX795 is nontoxic to HCE cells at therapeutic concentration.

Fig. 2 ITGA5 knockdown attenuates hPSC activation, differentiation, and functions. ITGA5 knockdown attenuates hPSC activation, differentiation, and functions.

Fig. 1 RNA methyltransferase METTL14 and demethylase ALKBH5 promote growth and invasion of breast cancer cells. RNA methyltransferase METTL14 and demethylase.

Fig. 2 Supraballs and films from binary SPs.

Fig. 5 Reconstitution of the dormant state using fetal ovaries.

Fig. 1 Schematic structure of a fluorescently labeled eGLP1-conjugated MALAT1 ASO and internalization of fluorescent eGLP1 and eGLP1-MALAT1-ASO. Schematic.

Fig. 1 ETL kills 4T1 breast carcinoma cells more efficiently than ST under static conditions. ETL kills 4T1 breast carcinoma cells more efficiently than.

Fig. 4 Systemic AAV9 delivery of gene editing components to ΔEx44 mice rescues dystrophin expression. Systemic AAV9 delivery of gene editing components.

Fig. 6 Pharmacologic alterations of β-AR signaling regulate cardiomyocyte abscission and endowment. Pharmacologic alterations of β-AR signaling regulate.

In vivo function of MeTro sealants using rat incision model of lungs

Fig. 3 Three small molecules inhibit the transport of CCR5 and do not affect transport of CCR1 or CXCR4. Three small molecules inhibit the transport of.

Fig. 3 Correction of Dmd exon 44 deletion in mice by intramuscular AAV9 delivery of gene editing components. Correction of Dmd exon 44 deletion in mice.

Fig. 2. Cellular response to FolamiRs in vitro.

The neuropod cells. The neuropod cells. (Top left) Neuropod cells synapse with sensory neurons in the small intestine, as shown in a confocal microscopy.

Fig. 4 Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. Rotavirus induces the type I IFN pathway and release of ATP by tumor.

Fig. 3 4n hybrids undergo bipolar and tripolar mitosis.

Presentation transcript:

Mammary cell proliferation is inhibited when LAD1 is depleted. Mammary cell proliferation is inhibited when LAD1 is depleted. (A) MTT analysis of viability in MCF10A cells expressing shLAD1 after 4 days in culture. Data are means ± SEM of six replicates. *P < 0.05, ***P < 0.001 (one-way ANOVA with Bonferroni’s correction). (B) Extracts of the indicated breast cancer lines were probed as indicated. (C) Serum-starved MDA-MB-231 cells expressing LAD1-V5 and shLAD1 MCF10A cells were stimulated with EGF (12 hours). Bromodeoxyuridine (BrdU) was added, and 60 min later, cells were stained. DAPI- and BrdU-stained nuclei were counted (in >5 fields). Data are means ± SEM of three experiments. *P < 0.05, ***P < 0.001. (D) MCF10A cells (5 × 103) stably expressing shLAD1 (or shScramble) were grown in EGF-containing Matrigel (2 weeks), acini were photographed, and ImageJ was used to determine volumes. ***P < 0.0001. Scale bar, 100 μm. Data are from three experiments. (E) MCF10A cells were incubated with RO-3306 (10 nM; 15 hours), washed, cultured (<90 min), and stained for DAPI, LAD1 (green), and acetylated tubulin (red). (F) Cells pretreated as in (D) were fixed and probed at metaphase. Scale bar, 10 μm. (G) Cells ectopically expressing LAD1-GFP and LifeAct-cherry were pretreated as in (D). Frames taken from live cell movies are shown. Scale bar, 10 μm. Lee Roth et al., Sci. Signal. 2018;11:eaan0949 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works