IL-10–treated dendritic cells decrease airway hyperresponsiveness and airway inflammation in mice Toshiyuki Koya, MD, PhD, Hiroyuki Matsuda, MD, PhD,

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Presentation transcript:

IL-10–treated dendritic cells decrease airway hyperresponsiveness and airway inflammation in mice Toshiyuki Koya, MD, PhD, Hiroyuki Matsuda, MD, PhD, Katsuyuki Takeda, MD, PhD, Shigeki Matsubara, PhD, Nobuaki Miyahara, MD, PhD, Annette Balhorn, BS, Azzeddine Dakhama, PhD, Erwin W. Gelfand, MD Journal of Allergy and Clinical Immunology Volume 119, Issue 5, Pages 1241-1250 (May 2007) DOI: 10.1016/j.jaci.2007.01.039 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Effect of transfer of IL-10+ DCs into naive mice before challenge. OVA-pulsed IL-10+ DCs (1 × 106), IL-10− DCs (1 × 106), or no cells were administered intratracheally to naive mice. Ten days after DC transfer, animals were challenged with OVA (1% in saline) for 20 minutes on 3 consecutive days. Forty-eight hours after the last OVA challenge, AHR was assessed, and BAL fluid and tissues were obtained for further analysis. Data represent means ± SEMs from 3 separate experiments (n = 12). A, Changes in lung resistance (RL). ∗∗P < .01 and ∗P < .05, comparing recipients of IL-10− DCs to IL-10+ DCs or no cells. B, Cellular composition in BAL fluid. Mac, Macrophages; Lym, lymphocytes; Neu, neutrophils; Eos, eosinophils. ∗P < .05 and ∗∗P < .01, comparing recipients of IL-10− and IL-10+ DCs or no DCs. C, Representative photomicrographs and quantitative analysis of PAS-positive cells in the lung tissues. The tissues were obtained 48 hours after the last challenge. Recipients of no cells (a), recipients of IL-10− DCs (b), recipients of IL-10+ DCs (c) are shown. Quantitative analysis of PAS-positive cells in lung tissues (d) was performed as described in the Methods section. ∗∗P < .01 comparing recipients of IL-10+ and IL-10− DCs or no DCs. BM, Basement membrane. Journal of Allergy and Clinical Immunology 2007 119, 1241-1250DOI: (10.1016/j.jaci.2007.01.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Effect of transfer of IL-10+ DCs into OVA-sensitized mice before airway challenge. OVA-pulsed or nonpulsed IL-10+ DCs or IL-10− DCs (1 × 106) were injected intravenously into OVA-sensitized mice before OVA challenge (OVA/OVA). Two days after DC transfer, animals were challenged with OVA (1% in saline) for 20 minutes on 3 consecutive days. Forty-eight hours after the last OVA challenge, AHR was assessed, and BAL fluid and tissue were obtained for further analysis. Control animals were nonsensitized but challenged with OVA (PBS/OVA). Data represent means ± SEM from 3 separate experiments (n = 12). A, Changes in airway resistance (RL). ∗P < .05 and ∗∗P < .01 between groups indicated. B, Cellular composition in BAL fluid. Mac, Macrophages; Lym, lymphocytes; Neu, neutrophils; Eos, eosinophils. ∗P < .05 between recipients of nonpulsed and pulsed IL-10+ DCs. C, Cytokine levels in BAL fluid. ∗P < .05 between recipients of nonpulsed and pulsed DCs. D, Representative photomicrographs and quantitative analysis of PAS-positive cells in the lung tissues. The tissues were obtained 48 hours after the last challenge. Shown are representative photomicrographs from the PBS/OVA group (a), OVA/OVA recipients of no cells (b), OVA/OVA recipients of non-pulsed IL-10+ DCs (c), and OVA/OVA recipients of pulsed IL-10+ DCs (d). Quantitative analysis of PAS-positive cells in lung tissues (e) is also shown. ∗P < .05 between recipients of nonpulsed and pulsed IL-10+ DCs. BM, Basement membrane. E, The percentage of IL-10–producing CD4+ T cells in the lung was determined by means of intracellular cytokine staining, as described in the Methods section. ∗P < .05 between recipients of nonpulsed and pulsed DCs. Journal of Allergy and Clinical Immunology 2007 119, 1241-1250DOI: (10.1016/j.jaci.2007.01.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Effect of transfer of IL-10+ DCs into OVA-sensitized mice before airway challenge. OVA-pulsed or nonpulsed IL-10+ DCs or IL-10− DCs (1 × 106) were injected intravenously into OVA-sensitized mice before OVA challenge (OVA/OVA). Two days after DC transfer, animals were challenged with OVA (1% in saline) for 20 minutes on 3 consecutive days. Forty-eight hours after the last OVA challenge, AHR was assessed, and BAL fluid and tissue were obtained for further analysis. Control animals were nonsensitized but challenged with OVA (PBS/OVA). Data represent means ± SEM from 3 separate experiments (n = 12). A, Changes in airway resistance (RL). ∗P < .05 and ∗∗P < .01 between groups indicated. B, Cellular composition in BAL fluid. Mac, Macrophages; Lym, lymphocytes; Neu, neutrophils; Eos, eosinophils. ∗P < .05 between recipients of nonpulsed and pulsed IL-10+ DCs. C, Cytokine levels in BAL fluid. ∗P < .05 between recipients of nonpulsed and pulsed DCs. D, Representative photomicrographs and quantitative analysis of PAS-positive cells in the lung tissues. The tissues were obtained 48 hours after the last challenge. Shown are representative photomicrographs from the PBS/OVA group (a), OVA/OVA recipients of no cells (b), OVA/OVA recipients of non-pulsed IL-10+ DCs (c), and OVA/OVA recipients of pulsed IL-10+ DCs (d). Quantitative analysis of PAS-positive cells in lung tissues (e) is also shown. ∗P < .05 between recipients of nonpulsed and pulsed IL-10+ DCs. BM, Basement membrane. E, The percentage of IL-10–producing CD4+ T cells in the lung was determined by means of intracellular cytokine staining, as described in the Methods section. ∗P < .05 between recipients of nonpulsed and pulsed DCs. Journal of Allergy and Clinical Immunology 2007 119, 1241-1250DOI: (10.1016/j.jaci.2007.01.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Effect of transfer of IL-10+ DCs from IL-10–deficient mice. A, Changes in lung resistance (RL) to inhaled MCh; B, BAL cellular composition. OVA-pulsed IL-10+ DCs from bone marrow of wild-type or IL-10–deficient mice were administered to OVA-sensitized mice before airway challenge. The data are expressed as means ± SEMs from 3 separate experiments (n = 12). ∗P < .05 or ∗∗P < .01 between recipients of DCs from IL-10+/+ and IL-10−/− donors. Journal of Allergy and Clinical Immunology 2007 119, 1241-1250DOI: (10.1016/j.jaci.2007.01.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions