POLYMERASE CHAIN REACTION

Slides:

Advertisements

Similar presentations

Structure of DNA. Polymerase Chain Reaction - PCR PCR amplifies DNA –Makes lots and lots of copies of a few copies of DNA –Can copy different lengths.

Advertisements

PCR way of copying specific DNA fragments from small sample DNA material "molecular photocopying" It’s fast, inexpensive and simple Polymerase Chain Reaction.

Enzyme names to learn 1.Reverse transcriptase 2.RNA polymerase 3.DNA helicase 4.DNA ligase 5.DNA polymerase 6.Restriction endonuclease A.Unwinds DNA helix.

The PCR The Polymerase Chain Reaction. The PCR is used to make copies of DNA (amplification). Whole genome OR DNA fragments.

The polymerase chain reaction (PCR) rapidly

ZmqqRPISg0g&feature=player_detail page The polymerase chain reaction (PCR)

DNA Replication DNA mRNA protein transcription translation replication Before each cell division the DNA must be replicated so each daughter cell can get.

Polymerase chain reaction

POLYMERASE CHAIN REACTION (PCR). THE TEMPLATE DNA GOES THROUGH A CYCLE OF HEATING AND COOLING TO FORM A NEW STRAND OF DNA THAT ONLY COMPRISES OF THE CANCEROUS.

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction Mrs. Stewart Medical Interventions.

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)

Module 1 Section 1.3 DNA Technology

What do these terms mean to you? You have 5 min to discuss possible meanings and examples with your group! DNA sequencing DNA profiling/fingerprinting.

Qai Gordon and Maddy Marchetti. What is Polymerase Chain Reaction? Polymerase Chain Reaction ( PCR ) is a process that amplifies small pieces of DNA to.

Chapter 14: DNA Amplification by Polymerase Chain Reaction.

Tina Doss Applied Biosystems

Success criteria - PCR By the end of this lesson we will be able to: 1. The polymerase chain reaction (PCR) is a technique for the amplification ( making.

Polymerase Chain Reaction Aims  To understand the process of PCR and its uses. Starter - Match each term with its correct description (work in pairs)

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction A process used to artificially multiply a chosen piece of genetic material. May also be known as DNA amplification. One strand.

Amplification of a DNA fragment by Polymerase Chain Reaction (PCR) Ms. Nadia Amara.

By: Cody Alveraz Ted Dobbert Morgan Pettit

PCR – Polymerase Chain Reaction A method of amplifying small amounts of DNA using the principles of DNA replication.

PCR Polymerase Chain Reaction. What is it and how is it useful?  A quick, easy method for amplifying (creating billions of copies) of unimaginably tiny.

FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT Chapter 13 Genetic Engineering Section 13-2 Manipulating DNA.

PCRPCR Presented by : Rana AL-Turki. 1- Definition of PCR. 2- Requirements for PCR. 3-PCR Process. 4-Procedure.

Introduction to PCR Polymerase Chain Reaction

Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase.

Lecturer: Bahiya Osrah Background PCR (Polymerase Chain Reaction) is a molecular biological technique that is used to amplify specific.

9.2 Copying DNA KEY CONCEPT The polymerase chain reaction rapidly copies segments of DNA.

Rajan sharma.  Polymerase chain reaction Is a in vitro method of enzymatic synthesis of specific DNA sequences.  This method was first time developed.

I. PCR- Polymerase Chain Reaction A. A method to amplify a specific piece of DNA. DNA polymerase adds complementary strand DNA heated to separate strands.

Section 4 Lesson 3– PCR. PCR – The Polymerase Chain Reaction PCR is a technique used to amplify specific sequences of DNA. This technique can be used.

Presented by: Khadija Balubaid.  PCR (Polymerase Chain Reaction) is a molecular biological technique  used to amplify specific fragment of DNA in vitro.

Success criteria - PCR By the end of this lesson we will be know:

SURVEY OF BIOCHEMISTRY Nucleic Acids continued… Amino Acids

Polymerase Chain Reaction (PCR)

PCR and Gel Electrophoresis

Do Now 3/13 Objectives: Describe the importance of PCR.

Polymerase Chain Reaction (PCR)

Molecular Cloning: Polymerase Chain Reaction

Presented by : Mostafa al-mosavi

Principle of PCR Principle of PCR Prof. Dr. Baron.

Polymerase Chain Reaction

Intro to PCR PCR (polymerase chain reaction) was invented by Kary Mullis in Mullis as a chemist working on small nucleotide strands for a biotech.

PCR uses polymerases to copy DNA segments.

Polymerase Chain Reaction & DNA Profiling

DNA profiling DNA profiling is a technique by which individuals can be identified and compared via their respective DNA profiles. Definitions you will.

16.3 – In vitro cloning Polymerase Chain Reaction

BIOTECHNOLOGY BIOTECHNOLOGY: Use of living systems and organisms to develop or make useful products GENETIC ENGINEERING: Process of manipulating genes.

3. PCR Page 376 – 377.

Polymerase Chain Reaction

PCR -PCR replicates (or amplifies) the DNA many times so that a large enough sample can be analyzed.

Polymerase Chain Reaction

POLYMERASE CHAIN REACTION (PCR): PRINCIPLES AND APPLICATIONS

Polymerase Chain Reaction (PCR)

Phenylthiocarbamide (PTC)

PCR uses polymerases to copy DNA segments.

PCR uses polymerases to copy DNA segments.

Introduction to Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)

PCR Polymerase chain reaction (PCR)

PCR uses polymerases to copy DNA segments.

PCR DNA fingerprinting Gel electrophoresis

3.5 Genetic modification and biotechnology

PCR uses polymerases to copy DNA segments.

PCR uses polymerases to copy DNA segments.

PCR uses polymerases to copy DNA segments.

Presentation transcript:

POLYMERASE CHAIN REACTION PCR © 2016 Paul Billiet ODWS

Features Amplifies small samples of DNA into millions of copies Can copy the DNA of a single cell Uses DNA polymerase from hot spring bacteria (Thermus aquaticus) Automated to produce millions of copies in a few hours. Taq DNA polymerase © 2016 Paul Billiet ODWS

Procedure Mix: DNA sample, specific complementary primers, DNA polymerase Heat DNA to 95°C, denatures DNA Sister strands separate Cool to 56°C Primers bind to DNA Bracket region to be copied Stop sister strand reannealing. © 2016 Paul Billiet ODWS

Procedure Warm to 73°C Optimum temperature for DNA polymerase New sister strands synthesised from the primers Heat again to 95°C Strands separate again Cool to 56°C more primers bind Warm to 73°C DNA polymerase synthesises new sister strands Repeat cycle 10 times > 1000 copies Repeat cycle 20 times > 1 million copies. © 2016 Paul Billiet ODWS

Problems Contamination of sample Any source of DNA will do (bacteria, technician…..) Sterile conditions needed. © 2016 Paul Billiet ODWS