Gel Electrophoresis Analysis

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Presentation transcript:

Gel Electrophoresis Analysis Using a DNA “ladder” composed of fragments of known length, you can determine the size of unknown DNA fragments

Typical Gel with HindIII Cut l Marker Measure Marker Fragments 1.8 cm 3.0 cm 3.6 cm 4.8 cm 7.8 cm 8.4 cm Measure Unknown Fragment of Interest Unknown Fragment 5.9cm

100 10 1 Fragment Length (Kb) 3.2 Kb 0 1 2 3 4 5 6 7 8 9 Fragment Migration Distance (cm) 5.9 cm

Using Excel Plot data in Excel semi-log chart Fit a power curve and display the equation for the fit Substitute unknown migration distance for X and solve