Fig. 4. A primary screen based on scrape closure

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Fig. 4. A primary screen based on scrape closure Fig. 4. A primary screen based on scrape closure.In controls, a scrape in a monolayer of 6TA2 cells, visible as the cell-free zone in a, is almost closed by 24 hours (b). A primary screen based on scrape closure.In controls, a scrape in a monolayer of 6TA2 cells, visible as the cell-free zone in a, is almost closed by 24 hours (b). In the presence of some inhibitors, scrape closure is greatly inhibited (c,d). The effect of a range of inhibitors is summarised in Table 1; the effects of inhibitors that focus on the Src–FAK system are shown quantitatively in (e). CK is Control Kidney medium, DMSO is the vehicle control, while the other labels are the names of drugs used at the concentrations shown in Table 1. Error bars show s.e.m; *indicates significance at P<0.05, **indicates P<0.01, by a t-test comparison with the control. Scale bars: 100 µm. Guangping Tai et al. Biology Open 2013;bio.20133780 © 2013. Published by The Company of Biologists Ltd