Fig. 3. Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3.

Slides:

Advertisements

Similar presentations

Identification of vegetally localised vrtn transcripts.

Advertisements

MiR-219 Regulates Neural Precursor Differentiation by Direct Inhibition of Apical Par Polarity Proteins Laura I. Hudish, Alex J. Blasky, Bruce Appel

Jeffrey D Amack, H.Joseph Yost Current Biology

Effect of cxcr3.2 mutation on dissemination of local mycobacterial infection within 24 hpi. Effect of cxcr3.2 mutation on dissemination of local mycobacterial.

Fig. 8. CCA and ChQ treatment induce accumulation of F-actin rings.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 4. smc3 regulates the expression of cx43 in regenerating fins.

Fig. 4. Abdominal aortic aneurysm formation in zebrafish embryos following treatment with Angiotensin II or snuff extract. Abdominal aortic aneurysm formation.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Fig. 2. Proportion of motile objects and track length

Altered oligodendrocyte progenitor cell (OPC) marker protein expression in two white matter regions of ShhΔFP/ΔFP and SmoΔNT/ΔNT embryos at E15.5. Altered.

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

Fig. 2. Long-term CCH increases SDH activity after 6 weeks and does not prevent an increase of the fibre cross-sectional area.Fish were kept for 3 or 6.

Fig. 1. Prestin in mouse outer hair cells is localized along the lateral wall of the cell along with β-catenin and Na/K ATPase.Shown are cartoons of the.

Fig. 1. Loss of PC following INT depletion

Fig. 1. Pigmentation and melanophore counts of rainbow trout parr and smolt caudal fins.Pigmentation of (A) parr and (B) smolt. Pigmentation and melanophore.

Fig. 1. γ-Tubulin localizes in close proximity to centriole walls in interphase but within an extended PCM meshwork in mitosis.U2OS cells were fixed and.

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

Fig. 3. Mutation of Y520 and Y667 result in increased delivery of prestin to the apical surface of MDCK cells.MDCK cells transiently transfected with wt.

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Fig. 1. Co-immunostaining of yeast spheroplasts with anti-hASNS and anti-alpha tubulin revealed that yeast asparagine synthetases formed filaments/foci.

Fig. 4. Non-autonomous rescue of puc expression in DME cells

Elevated pAkt level in ptena−/−ptenb−/− mutants is suppressed by LY and to a lesser extent by sunitinib. Elevated pAkt level in ptena−/−ptenb−/−

Wound recruitment of melanocytes requires innate immune cells.

Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 6. Localization of Sma during growth and migration

Fig. 2. Morphological analysis of acentriolar mitotic spindles

Fig. 7. Lhx1-RNAi reduces the eye size

Fig. 2. Centrosomal proteins display distinct localizations and radial distances from centriole walls.U2OS cells were fixed and stained with the indicated.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Fig. 4. Co-immunostaining of nocodazole or ASNase treated RPE-1 cells with anti-hASNS and anti-alpha tubulin showed defect in both mitotic spindle formation.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

Fig. 3. Reduced histone H3K9 trimethylation in SCNT embryos treated with TSA and VC. (A) Immunostaining analysis indicated that SCNT embryos with TSA+VC.

Fig. 2. TSA impairs growth and disrupts morphogenesis of exocrine pancreas in zebrafish larvae with hyperacetylation of nucleosomal histones.WT zebrafish.

Fig. 8. The morphology of the ventral nerve cord in Ror-Myc overexpressing embryos is normal. The morphology of the ventral nerve cord in Ror-Myc overexpressing.

prdm1a is co-expressed with foxd3 and tfap2a at the NPB

Fig. 9. Mip120 CXC and HCH domains are not sufficient for nuclear localization in mip120 null egg chambers. Mip120 CXC and HCH domains are not sufficient.

Fig. 2. Immunostaining for ClC-5 in isolated hog gastric mucosa

Fig. 5. Upregulation of N. vectensis thrombospondin during regeneration.(A,B) Juvenile polyps were fixed 48 hours following transection and processed.

Fig. 3. Exogenous folic acid rescues neural epithelial apical constriction and activation of non-muscle myosin upon Rho-kinase inhibition. Exogenous folic.

Fig. 4. Perinuclear dynein regulators are not required for primary ciliogenesis.RPE cells were transfected with siRNA and either nocodazole-treated, fixed,

Fig. 1. Loss of PC following INT depletion

Fig. 3. Enhanced EGFR signaling in Rhbdf2P159L/P159L mice.

Folic acid increases apical junctional pMLCK localization in vivo

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

Fig. 2. Non-homogeneous subcellular distribution of Vangl2 along the anteroposterior axis. Non-homogeneous subcellular distribution of Vangl2 along the.

Fig. 2. iPSCs produce functional osteoblasts.

Effects of Chd1-knockdown on Hmgpi and Klf5 expression

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

Zebrafish as a Model Organism for the Identification and Characterization of Drugs and Genes Affecting p53 Signaling Ulrike Langheinrich, Elisabeth Hennen,

Fig. 2. Acetylation stiffens primary cilia.

Fig. 1. Rnd2 and Rnd3 induce stress fibres whereas Rnd1 reduces stress fibres in endothelial cells.(A) Rnd mRNAs are expressed in HUVECs. Total RNA was.

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

Fig. 3. MO-mediated smc3 knockdown results in reduced regenerate length, segment length and cell proliferation. MO-mediated smc3 knockdown results in reduced.

VIP treatment decreased the number of apoptotic cells and increased cell proliferation. VIP treatment decreased the number of apoptotic cells and increased.

mip120 null egg chambers have a condensed nurse cell DNA phenotype

Fig. 6. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. Bj mutants show stereocilia patterning defects and biliary.

Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.

Fig. 1. Lhx1 is expressed in the proximal region of the OV

CBFβ deficiency leads to heart failure in zebrafish.

Fig. 4. Tetracycline-regulated expression of ClC-5 in the HEK293 cells stably expressing gastric H+,K+-ATPase.(A) Alignments of rat ClC-5, human ClC-5,

Rab18−/− mice have large accumulations of neurofilament at the neuromuscular junction. Rab18−/− mice have large accumulations of neurofilament at the neuromuscular.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Co-expression of periostin and Sox10 in DRG and DRG explant cultures.

Aplf knockdown does not compromise DNA repair competency in iPSCs.

Ctip2 directly regulates Notch1 expression in differentiated cells.

Tumors treated with anti-Flk-1 mAb have increased tumor cell apoptosis, reduced tumor cell proliferation, and increased tumor necrosis. s.c. Tumors treated.

Presentation transcript:

Fig. 3. Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3 MO (D–F), cited3 MO + cited3 RNA (G–I) were fixed at 2 dpf and immunostained with anti-Mef2 antibody (A,D,G) and TMR red in situ cell death detection kit (B,E,H). Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3 MO (D–F), cited3 MO + cited3 RNA (G–I) were fixed at 2 dpf and immunostained with anti-Mef2 antibody (A,D,G) and TMR red in situ cell death detection kit (B,E,H). Tunel positive cells were counted in non-muscle cells as well as in muscle cells in each group of embryos from 50–100 sections obtained from trunk and tail somites. More apoptotic muscle cells were detected in embryos that are injected with the cited3 MO and fixed at 2 dpf and 3 dpf (M). Values were normalized compared to cited3 morphant values. Similarly, embryos injected with the control MO (J), cited3MO (K), cited3 MO + cited3 RNA (L) was pulsed with BrdU from 30 hpf to 42 hpf, and were fixed and immunostained with anti-BrdU and MF20 antibodies (images of 16th–18th somites). There is no significant difference between the three treated groups (J,K,L) when the BrdU-positive muscle cells were counted (data not shown). Scale bars: 25 µm in A and 50 µm in K. Gnanapackiam Sheela Devakanmalai et al. Biology Open 2013;2:505-514 © 2013. Published by The Company of Biologists Ltd