Fig. 6. The cholinergic receptor subunits a6 (Chrna6) and b3 (Chrnb3) are (subset) specifically expressed in mdDA neurons during development.(A) The Chrna6.

Slides:

Advertisements

Similar presentations

Immunohistochemistry and in situ hybridization allow researchers to pinpoint the expression of their protein and nucleic acid targets, respectively.

Advertisements

Fig. 1. In situ hybridization analysis for detecting endogenous mBest1 transcript in whole-brain region. In situ hybridization results from coronal (top.

Identification of vegetally localised vrtn transcripts.

Fig. 2. Effect of endurance training on gene expression, and protein content and activity in heart muscle. Effect of endurance training on gene expression,

Fig. 2. Outline of the two types of stimulus sequences employed in the analysis.(A) Environment information stimuli; (B) adaptation stimuli. Outline of.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 1. Muscle-specific PITX1 over-expression in the Pitx1 transgenic mice.(A) Detection of Pitx1 mRNA expression in muscles of the Pitx1 transgenic mice.

Fig. 6. Comparison between the response against transformed tissues and capsule formation.At the cellular level the two responses share many similarities.

Fig. 4. Induction of a systemic immune response in RasV12-expressing larvae.(A) List of genes that are significantly induced (at q

Fig. 2. Long-term CCH increases SDH activity after 6 weeks and does not prevent an increase of the fibre cross-sectional area.Fish were kept for 3 or 6.

Fig. 2. Body weight and size analysis of A1/A2-KO mice

Fig. 5. Onecut transcription factors are important for the correct generation of the mdDA neuronal population.(A) Schematic representation of the region.

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Table 2. Cell surface abundance of β1 integrin measured by flow cytometry.DDR wild type and DDR over-expressing cells were treated with deoxymannojirimycin.

Fig. 1. Pigmentation and melanophore counts of rainbow trout parr and smolt caudal fins.Pigmentation of (A) parr and (B) smolt. Pigmentation and melanophore.

Fig. 7. E2F1 acetylation in A1/A2-KO MEFs

Fig. 4. Targeted disruption of STK35 transcripts in mouse.

Fig. 7. VCBP-C and chitin localization in stomach sections of adult DSS- and CMP-treated animals. VCBP-C and chitin localization in stomach sections of.

Increased osteoclastogenesis in the mandibular bone of Fgfr2+/S252W embryos at E16.5. Increased osteoclastogenesis in the mandibular bone of Fgfr2+/S252W.

Fig. 5. GFP fluorescence colocalization of Gcn5.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

Fig. 4. BKA values for different species.

Fig. 1. Generation of WNK3 knockout mice

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

Fig. 4. Co-immunostaining of nocodazole or ASNase treated RPE-1 cells with anti-hASNS and anti-alpha tubulin showed defect in both mitotic spindle formation.

Fig. 2. STK35 RNAs are expressed in distinct yet overlapping cell populations in adult mouse testis. STK35 RNAs are expressed in distinct yet overlapping.

Fig. 6. STK35 KO mice show ovary defects.

Distribution and extent of expression.

Fig. 2. TSA impairs growth and disrupts morphogenesis of exocrine pancreas in zebrafish larvae with hyperacetylation of nucleosomal histones.WT zebrafish.

GABAergic subtype switch in the absence of Gata2 in the pTh-R precursors. GABAergic subtype switch in the absence of Gata2 in the pTh-R precursors. (A-T)

Statistical chart of significantly differentially expressed genes

The genomic distribution of essential and non-essential mouse genes, separated into known and predicted essentiality. The genomic distribution of essential.

prdm1a is co-expressed with foxd3 and tfap2a at the NPB

Fig. 1. Expression of the five miRNAs encoded by two miRNA clusters in mouse sperm and oocytes.(A) qPCR analyses of levels of miR-16 (positive control),

Fig. 2. Sufficient rate of recombination in embryonic NSCs and NPCs of Emx1cre knock-in mice.(A) Breeding schemes to generate mice with the Emx1cre locus.

Fig. 11. Chrna6 and Chrnb3 expression depends Pitx3 and Nurr1 but through a different mechanism reported for Vmat2.(A) QPCR analysis of Chrna6 and Chrnb3.

Fig. 4. Expression analysis of Onecut transcription factors during mdDA neuron development.Adjacent coronal sections of E11.5, E12.5 and E13.5 mouse brains.

Fig. 10. Expression of the cholinergic receptor subunits Chrna6 and Chrnb3 depends on Pitx3.In situ hybridization for Th, Chrna6 and Chrnb3 on sagittal.

Conserved patterns of Shh expression from mammals to teleosts.

Fig. 6. The cholinergic receptor subunits a6 (Chrna6) and b3 (Chrnb3) are (subset) specifically expressed in mdDA neurons during development.(A) The Chrna6.

Fig. 1. Insufficient rate of recombination in NSCs and NPCs of Nestin-cre transgenic mice.(A) Breeding schemes to generate Nestin-cre transgenic mice (Jax:

Fig. 5. Upregulation of N. vectensis thrombospondin during regeneration.(A,B) Juvenile polyps were fixed 48 hours following transection and processed.

Fig. 9. Loss of the cholinergic receptor subunit Chrna6 does not alter the development and organization of the mdDA system.(A) In situ hybridization experiments.

Fig. 5. Flatworm density and activity on coral polyps

Fig. 2. Tbx1 lineage is largely complementary to the NSD

Stage-specific expression modules of preimplantation development.

Fig. 4. Inactivation of Tbx1 and Jag1 with Tbx1Cre results in expanded proneural gene expression. Inactivation of Tbx1 and Jag1 with Tbx1Cre results in.

Amplicon sequencing analysis of on-target sites in trβ crispants

Fig. 6. IIS affected the soma area of most neurons in pharate adults, but not in larvae.(A,B) Pan-peptidergic expression pattern of 386-Gal4,UAS-CD8::GFP.

Fig. 12. Overview of the molecular program essential to build mdDA neurons.The genes identified in this study (in red) have been added to the programming.

Effects of Chd1-knockdown on global gene expression.

The zebrafish swdp75fm and swdp82mf mutations cause exocrine pancreatic hypoplasia and reduced skin pigmentation. The zebrafish swdp75fm and swdp82mf mutations.

Fig. 2. Expression of Cx43 mutant T154A resulted in non-radial spreading and formation of protrusions in J558µm3 cells spreading in response to BCR signaling.(A)

Fig. 1. Expression of the five miRNAs encoded by two miRNA clusters in mouse sperm and oocytes.(A) qPCR analyses of levels of miR-16 (positive control),

Expression of smc3 in whole-mount and cryosectioned regenerating fins

Fig. 7. Eye defects in STK35 KO mouse.

Fig. 3. Mean force and velocity during jumping

Fig. 2. Temporal map of genes clustering with the expression profile of Lmx1a.(A) Heat-map visualization obtained by HCL of genes clustering with Lmx1a.

Fig. 1. Microarray analyses of genes whose expression is regulated by innervation during synaptogenesis.(A) Schematic drawings of the experimental design.

Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.

Table 1. Measurement of ring diameters of proteins localizing in ring-like patterns around centrioles.Consideration of the size of IgG (about 8 nm) raises.

Fig. 1. Lhx1 is expressed in the proximal region of the OV

Fig. 5. Behaviours of the wild-types Oregon-R at two temperatures.

Phenotypic analysis of the CNS in mutants for Ror, otk and otk2

Fig. 8. Expression of other genomic-clustered Chrn subunits in the mesodiencephalon.(A) Schematic representation illustrating the assembly of the Chrnb4,

Identification of a novel subset of mDA neurons in the VTA that expresses Neurod6, OTX2, CALBINDIN1, ALDH1A1, and Grp. Identification of a novel subset.

Selective expression of Neurod6 in mDA neurons in the VTA

Presentation transcript:

Fig. 6. The cholinergic receptor subunits a6 (Chrna6) and b3 (Chrnb3) are (subset) specifically expressed in mdDA neurons during development.(A) The Chrna6 and Chrnb3 genes are organized as a tandem in the mouse genome (chromosome 8) in a tail to tail orientation. The cholinergic receptor subunits a6 (Chrna6) and b3 (Chrnb3) are (subset) specifically expressed in mdDA neurons during development.(A) The Chrna6 and Chrnb3 genes are organized as a tandem in the mouse genome (chromosome 8) in a tail to tail orientation. (B) In situ hybridization (ISH) experiments for Chrna6, Chrnb3 and Th (position control) on sagittal sections of E13.5 mouse brain. The expression patterns of Chrna6 and Chrnb3 are restricted to the mdDA neuronal region and appear to be restricted to a subset of mdDA neurons. (C) Combined ISH (purple) and immunohistochemistry (brown) experiment using Chrna6 and Chrnb3 probes with Th antibodies confirm the co-localization and restriction to the mdDA neuronal pool of the Chrna6 and Chrnb3 transcripts. L, lateral; M, medial. Koushik Chakrabarty et al. Biology Open 2012;bio.20121230 © 2012. Published by The Company of Biologists Ltd