A subset of Trp53CKO fiber cells fail to exit the cell cycle, but express fiber-cell-specific markers. A subset of Trp53CKO fiber cells fail to exit the.

Slides:

Advertisements

Similar presentations

From: A Hierarchy of Proliferative Cells Exists in Mouse Lens Epithelium: Implications for Lens Maintenance Invest. Ophthalmol. Vis. Sci ;47(7):

Advertisements

Primary culture of mouse middle ear epithelial cells.

Ocular defects produced by Daam1 mutation and in combination with diabetes. Ocular defects produced by Daam1 mutation and in combination with diabetes.

Frozen sections of (A) fetal intestine, (B) tonsil, and (C) mantle cell lymphoma, stained using anti-CD3 (immunoperoxidase; brown) followed by anti-CD5.

Percentage fractions of (β-Gal+) PCs in 32-week-old heterozygous females differ between cerebellar lobules affected and spared from degeneration of Purkinje.

Inducible DM1 model displays increased autophagy.

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Nephrogenesis in vitro can be rescued in 3D co-cultures by siRNA treatment of Renca cells. Nephrogenesis in vitro can be rescued in 3D co-cultures by siRNA.

Aberrant development of the anterior segment in AP-2β NCC KO mutants.

Total and differential cell counting in the broncho-alveolar lavage of mice exposed to CS or air during 3 or 6 months. Total and differential cell counting.

Localisation of epithelial markers in mMEC cultures.

Notch pathway components in serial sections of the human colon.

Remodeling and maturation of the mouse retinal vasculature.

X-ray radiographic images showing structural alterations of the joints that are most commonly affected by OA. (A) Normal and (A′) severely affected joint.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

Enterocytes fail to migrate consistently towards the villus tip in live APCMin/+ tissue. Enterocytes fail to migrate consistently towards the villus tip.

Hipk induces cell spreading.

Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.

Ectopic sprouting and proliferation in Rbpj veins.

Challenge with oxazolone results in epithelial hyperplasia and influx of inflammatory cells into the epidermis and dermis and keratosis as observed in.

Hypoacidity, anemia and hypergastrinemia in KI/KI mice.

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

Expression data confirm and extend existing knowledge

Α-Catenin DKO cardiomyocytes exhibit increase proliferation on stiff ECM. (A) Representative images of neonatal cardiomyocytes cultured on different PA-FN.

Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. Effects of doxorubicin.

Morphological defects of hi559 GI tract.

Adult cardiomyocyte renewal via deletion of Hippo pathway genes.

Transcription factor network of human pancreas development.

In 6CFSMEo- cells, UPF1 protein is concentrated partially or totally in P-bodies after cytoskeleton inhibitor treatment. In 6CFSMEo- cells, UPF1 protein.

G418 causes UPF1 to localize to cytoplasmic foci containing NMD substrates but not the P-body marker DCP1a. 6CFSMEo- cells were transfected with constructs.

Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen.

Cre-loxP-mediated chromosomal engineering in mice.

The effect of TDP-43A315T expression on dendrite spine development.

Distribution and extent of expression.

Functional classification and visualization of differentially expressed genes. Functional classification and visualization of differentially expressed.

GABAergic subtype switch in the absence of Gata2 in the pTh-R precursors. GABAergic subtype switch in the absence of Gata2 in the pTh-R precursors. (A-T)

TWEAK increases histone crotonylation in kidney tubular cells.

The effect of TDP-43A315T expression at the synapse.

2DG downregulates the expression of HIF-1α, PDK1 and c-Myc in NB xenograft. 2DG downregulates the expression of HIF-1α, PDK1 and c-Myc in NB xenograft.

Rgs16::GFP expression in pancreatic neoplasia.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Z-stack compressions showing triple labelling of different cell types in the gills of bowfin with serotonin antibody (5-HT, green) and the neuronal marker.

Homozygous col22a1−/− embryos show increased percentage of hemorrhages when raised at increased temperature. Homozygous col22a1−/− embryos show increased.

Overexpression of Lef-1 partially rescues tooth arrest in Sox2cKO embryos. Overexpression of Lef-1 partially rescues tooth arrest in Sox2cKO embryos. (A,B)

Injection volume influences tumor architecture and desmoplastic response. Injection volume influences tumor architecture and desmoplastic response. (A)

Loss or increase of Tj causes a reduction in Slbo expression.

Sox2 regulates dental epithelial stem cell proliferation and differentiation. Sox2 regulates dental epithelial stem cell proliferation and differentiation.

Effect of Dipy on the expression of chondrogenic markers.

Fig. 3. Enhanced EGFR signaling in Rhbdf2P159L/P159L mice.

Z-stack compressions showing triple labelling of different cell types in the gills of bowfin with antibodies for serotonin (5-HT, green) and HNK-1 (magenta),

Shp2 is required for lens and lacrimal gland development.

Organoid-derived transplantable corneal epithelial grafts on human amniotic membrane substrate. Organoid-derived transplantable corneal epithelial grafts.

IGF1R is present in endometrial luminal epithelium at the receptive phase. IGF1R is present in endometrial luminal epithelium at the receptive phase. Endometrial.

IHC characterization of 15-week-old mature corneal organoid in suspension. IHC characterization of 15-week-old mature corneal organoid in suspension. Confocal.

The expression of two forms of N-cadherin.

Peroxisome speeds were slower in patient and control cells.

Immunofluorescence staining of Gli in the osmoregulatory tissues of Aedes aegypti larva. Immunofluorescence staining of Gli in the osmoregulatory tissues.

Integrins regulate PFC maturation by regulating dap expression.

Adult Acvr1;Trp53DCKO lenses form vascularized, tumor-like structures at their posterior poles. 4-month-old Trp53CKO and Acvr1;Trp53DCKO lenses were sectioned.

Proliferative and morphological defects following TbCALM and TbEpsinR depletion. Proliferative and morphological defects following TbCALM and TbEpsinR.

Lack of Ctip2 is associated with impaired proliferation and delayed onset of differentiation during early stages of epidermal development. Lack of Ctip2.

Aplf downregulation does not induce cellular arrest.

BMOL3 LPCs can differentiate towards the hepatocyte lineage.

Disruption of the tubule formation by immortalized cells during nephrogenesis in vitro. Disruption of the tubule formation by immortalized cells during.

DBZ treatment can induce virtually complete exfoliation of BE epithelium. DBZ treatment can induce virtually complete exfoliation of BE epithelium. (A,B)

2DG suppresses of lamellipodia and filopodia and causes disorganization of F-actin filaments in murine endothelial cells. 2DG suppresses of lamellipodia.

Upon challenge with oxazolone, human leukocytes (mainly consisting of T cells) infiltrate the dermis and epidermis. Upon challenge with oxazolone, human.

Volume 53, Issue 4, Pages (April 1998)

Presentation transcript:

A subset of Trp53CKO fiber cells fail to exit the cell cycle, but express fiber-cell-specific markers. A subset of Trp53CKO fiber cells fail to exit the cell cycle, but express fiber-cell-specific markers. P3 lenses were stained with antibodies that label markers of cell proliferation, cell cycle exit and lens fiber cell differentiation. (A) Labeling for Ki67, a marker for actively cycling cells, is restricted to the anterior epithelium of Trp53WT lenses. (B) In Trp53CKO lenses, a few cortical fiber cells are Ki67 positive (arrowheads). (C) BrdU is only incorporated into anterior epithelial cells in Trp53WT lenses. (D) A few Trp53CKO fiber cells are BrdU positive (arrowheads). (E) p57KIP2, a marker of cell cycle exit, is strongly expressed in all nuclei in the lens equator of Trp53WT lenses. (F) Most cells in Trp53CKO lenses have nuclei that are stained with antibody to p57KIP2, but a few nuclei are p57KIP2 negative (arrow). (G) Ki67 labeling is lost near the equator, where the fiber-specific transcription factor, Maf (c-Maf) is first expressed. (H) Fiber cells in Trp53CKO lenses express Maf, although some are also Ki67 positive (arrowhead). (I) MIP, a major component of fiber cell membranes, is strongly expressed throughout the fiber zone of Trp53WT lenses. (J) MIP is strongly expressed in Trp53CKO fiber cells, even when they express Ki67 (arrowheads). Luke A. Wiley et al. Dis. Model. Mech. 2011;4:484-495 © 2011. Published by The Company of Biologists Ltd