Fig. 2. p85β regulates cell adhesion

Slides:

Advertisements

Similar presentations

ABAE positive cells 6h after treatment

Advertisements

miR-133a positively regulated p53/p21 pathway.

An siRNA screen identifies LIMT as an ERK‐dependent negative regulator of mammary cell migration An siRNA screen identifies LIMT as an ERK‐dependent negative.

PDGFRβ is dispensable for EGFRvIII-driven GBM growth but is required for the optimal growth of EGFR-inhibited tumors. PDGFRβ is dispensable for EGFRvIII-driven.

FAS1 domain protein inhibits association of αvβ3 integrin with VEGFR-2 and attenuates VEGF165-induced VEGFR-2 phosphorylation. FAS1 domain protein inhibits.

A20 inhibits caspase-8 cleavage and TRAIL-induced apoptosis.

Volume 27, Issue 5, Pages (November 2007)

XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells.

TRAF4 is required for EGFR activation in response to EGF stimulation.

TGF-β stimulates SMAD7 expression via FOXO3.

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Fig. 4. Clinically defined SMN mutants show alterations in the association with telomerase complex proteins. Clinically defined SMN mutants show alterations.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

TC-1 silencing sensitized A549 and SPC-A-1 cells to radiation therapy

Specific inhibition of Ras, but not Rac, inhibits EGF-stimulated protrusion. Specific inhibition of Ras, but not Rac, inhibits EGF-stimulated protrusion.

Table 1. Menthol preference index (MPI) and average number of eggs laid per female (EPF) in F0 and F1 lines.The statistical significance of MPI was assessed.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr.

Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Fig. 1. Loss of PC following INT depletion

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Fig. 8. p85β regulates active Cdc42 and Rac localization to deep adhesions.(A) NIH3T3 cell lines expressing p85α or p85β were incubated without serum (2 h),

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 1. Coilin interaction with the telomerase complex protein dyskerin is mediated by hTR. Coilin interaction with the telomerase complex protein dyskerin.

Table 2. Cell surface abundance of β1 integrin measured by flow cytometry.DDR wild type and DDR over-expressing cells were treated with deoxymannojirimycin.

Abraxas binds to BRCT–PALB2(L21P) and is involved in the recruitment of this fusion protein or of endogenous PALB2 to sites of DNA damage. Abraxas binds.

eb1a-2 eb1b-3 double-mutant plants display skewed and shorter roots

Fig. 7. E2F1 acetylation in A1/A2-KO MEFs

Fig. 7. SCF and IGF-1 accelerate fetal spleen erythropoiesis in vivo.

Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin.

Doxorubicin viability in the presence and absence of Herceptin

Fig. 7. ML mediated suppression of SAHA-induced up-regulation of tRNA as a potential mechanism that contributes to the enhanced anti-cancer actions.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

FAK tyrosine phosphorylation is unaffected by loss of LAR activity.

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

Fig. 4. Coilin phosphomutant displays differential RNA binding and degradation activities.Purified proteins were analyzed for RNase activity with total.

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

Fig. 4. Co-immunostaining of nocodazole or ASNase treated RPE-1 cells with anti-hASNS and anti-alpha tubulin showed defect in both mitotic spindle formation.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

Fig. 5. Combination of SAHA and ML produces augmented suppression of cellular proliferation with impaired cell cycle progression, enhanced apoptotic.

Fig. 2. Kinetics of OptoEphB2 activation.

Reb does not play a significant role in regulating TGF-β signaling

Fig. 4. Elevated levels of tRNAiMet promote migration and invasion of melanoma cells. Elevated levels of tRNAiMet promote migration and invasion of melanoma.

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Macrophage-produced IL-10 is required but not sufficient for macrophage-stimulated proliferation of ADPKD cells. Macrophage-produced IL-10 is required.

Interaction of periostin with ECM molecules.

Fig. 1. Loss of PC following INT depletion

The K86M mutation in ABCG2 results in a non-functional transporter.

The SIM domain of RAD51 is required for HR

Defective MEK-Erk1/2 kinase activation in RIAM-depleted cells accounts for their impaired FA disassembly. Defective MEK-Erk1/2 kinase activation in RIAM-depleted.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Synergistic WNT1 and WNT7B signaling is downstream of LRP6 phosphorylation and β-catenin stabilization. Synergistic WNT1 and WNT7B signaling is downstream.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Regulation of lipid droplet formation by PI3-kinase activity.

Analysis of mutant Best1 protein (Y227N) in testis and sperm function

Kinetics of GFP secretion from CHO cells.

miR-145 overexpression reduces IGF1-coated bead attachment.

Integrin β3 is dispensable for RhoA activity and contractility.

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

Fig. 1. p85β localizes at adhesion plaques and associates with FAK

Elevated levels of tRNAiMet promote α5β1-dependent cell migration

Fig. 7. Nrf2-dependent enzyme activities in wild-type, Nrf2- and Keap1-deficient tissues.Hepatic (A,C,E) and cortical (B,D,F) enzyme activities of NQO1.

Fig. 4. Tetracycline-regulated expression of ClC-5 in the HEK293 cells stably expressing gastric H+,K+-ATPase.(A) Alignments of rat ClC-5, human ClC-5,

Fig. 1. lgl interacts genetically with Argonaute 1 (AGO1) in the eye.

BCL-3 regulates expression of stemness-associated Wnt targets.

Volume 10, Issue 9, Pages (March 2015)

Engagement of PDGF-αR and PDGF-βR is critical for PDGF-BB-mediated increased proliferation of NPCs. RT-PCR (A) and WB (B) of PDGF-αR and PDGF-βR in NPCs;

Vps36 interacts with Smo in the absence of Hh

Induction of CDCP1 is regulated by Ras in NSCLC cells.

Presentation transcript:

Fig. 2. p85β regulates cell adhesion Fig. 2. p85β regulates cell adhesion.(A) p85α or p85β expression was analyzed in WB in stably transfected NIH3T3 cells. p85β regulates cell adhesion.(A) p85α or p85β expression was analyzed in WB in stably transfected NIH3T3 cells. Freshly isolated MEF (from WT, p85α−/− or p85β−/− mice) were genotyped by PCR. A fraction of the cells were activated with PDGF (50 ng/ml) and used in an adhesion assay, alone or on fibronectin (FN), or in a migration assay on transwells coated with a thin layer of FN or collagen (COL)(10 µg/ml). Graphs show the percent of response relative to the maximal in control cells (100%; mean ± s.d.; n = 5). (B) Control and p85β-overexpressing NIH3T3 cells were transfected with FAK siRNA (48 h) or preincubated with FAK inhibitor (10 µM PF-573228) for 1 h at 37°C prior to stimulation with 50 or 100 ng/ml PDGF (indicated) on FN. The cells were assayed in an adhesion assay (as in A). Some of the cells transfected with siRNA or treated with the inhibitor were lysed and lysates examined in WB using were anti-FAK Ab or anti-pTyr576/577-FAK Ab (indicated). n.s. = non-statistical significance; ** P<0.01, * P<0.05; Student's t-test. Ariel E. Cariaga-Martínez et al. Biology Open 2014;bio.20148185 © 2014. Published by The Company of Biologists Ltd