MicroCT analysis reveals that IHBD average volume is modulated with age upon deletion of RBP-J or activation of Notch1. MicroCT analysis reveals that IHBD average volume is modulated with age upon deletion of RBP-J or activation of Notch1. Reconstruction of microCT scans provides a high-resolution global representation of resin casts. (A) Representative image from Scanco microCT 40 scanning of P120 control IHBD resin cast at 20 μm resolution. Red square demonstrates area in A’. (B) Total IHBD cast volume was calculated using software adapted from trabecular bone analysis (see Methods). At P60, RBP KO cast volume is equivalent to control; however, as age increases, the volume decreases when compared with control IHBD casts. This decrease is statistically significant at P120. The trend in NICD IHBD casts shows an increase in cast volume. For each genotype (control, RBP KO, NICD), casts at P60 (n=4, 5 and 3, respectively), P90 (n=4, 6 and 3, respectively) and P120 (n=4, 4 and 3, respectively) were scanned. Error bars represent s.e.m. *P≤0.05, **P≤0.01. Scale bars: 1 mm. Erin E. Sparks et al. Dis. Model. Mech. 2011;4:359-367 © 2011. Published by The Company of Biologists Ltd