Fig. 1. Lipid analysis.(a) HP-TLC analysis of DIMs of tobacco pollen tubes, using increasing detergent/protein ratios. Lipid analysis.(a) HP-TLC analysis.

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Fig. 1. Lipid analysis.(a) HP-TLC analysis of DIMs of tobacco pollen tubes, using increasing detergent/protein ratios. Lipid analysis.(a) HP-TLC analysis of DIMs of tobacco pollen tubes, using increasing detergent/protein ratios. The graph shows the percentages of different lipid species with respect to P2. The analysis refers to at least three independent preparations. A significant increase in total sterols and glucosylceramide (GluCer) is evident at ratio 8:1. The percentage remains constant at higher ratios of 10:1 and 12:1 (light blue and plum lines, respectively) while percentages of glycerophospholipids, such as phosphatidylcholine (PC, blue line), phosphatidylethanolamine (PE, red line) and other phospholipids (green line), decrease. (b) Analysis of hydroxylated/non-hydroxylated VLCFAs. The percentage increases significantly in DIMs with respect to P2 at detergent/protein ratios of 8:1 and 12:1 (mean of two experiments with similar values). (c) The graph shows the increased ratio of saturated/unsaturated phospholipid fatty acids in DIMs with respect to P2 at increasing detergent/protein ratios. The percentage increases up to ratio 7:1 and remains constant at ratios 8:1–12:1 (n = 3). Error bars indicate standard errors. Alessandra Moscatelli et al. Biology Open 2015;bio.201410249 © 2015. Published by The Company of Biologists Ltd