Low spatial resolution SIMS images of a 2 μm-thick, freeze-substituted section of Galaxea fascicularis showing the distribution of (A) 40Ca and (B) 44Ca.

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Low spatial resolution SIMS images of a 2 μm-thick, freeze-substituted section of Galaxea fascicularis showing the distribution of (A) 40Ca and (B) 44Ca after 1 min incubation in 44Ca artificial seawater in the light. Low spatial resolution SIMS images of a 2 μm-thick, freeze-substituted section of Galaxea fascicularis showing the distribution of (A) 40Ca and (B) 44Ca after 1 min incubation in 44Ca artificial seawater in the light. The 44Ca/40Ca ratio image (C) and line profile (E) measured over A–B in (C) indicate that relatively little 44Ca had entered the cells and extrathecal coelenteron, with the highest relative concentration being in the external seawater layer. Grey values in E represent pixel intensity values along A–B in (C) as a function of distance (μm). (D) Adjacent section, stained with Toluidine Blue, showing the external seawater layer (SW), oral ectoderm (OE), mesogloea (M), oral gastrodermis (OG), extrathecal coelenteron (ETC), aboral gastrodermis (AG) and calicoblastic ectoderm (CE). Alan T. Marshall et al. J Exp Biol 2007;210:2453-2463 © The Company of Biologists Limited 2007