Fig. 4. Effect of royal jelly on silkmoth fat body cells and eggs.

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Fig. 4. Effect of royal jelly on silkmoth fat body cells and eggs. Effect of royal jelly on silkmoth fat body cells and eggs. (A) First instar silkmoth larvae were reared to adult moths by feeding artificial diets supplemented with 5.6% w/w royal jelly (RJ) or without royal jelly (Basal) throughout the larval stage. Fat body cells from male silkmoths were observed by microscopy and the cell area was measured as described in the Materials and Methods. The vertical axis indicates the cell area (µm2). All data are plotted (each dot represents each individual) in the graph with boxplots showing quartiles. Asterisk indicates a significant difference compared to the Basal diet group in the Wilcoxon rank-sum test (P=5.36×10−4). (B-D) Eggs were collected from the silkmoth abdomen on the day of adult emergence, and the number (B), total mass (C), and mass per egg (D) were calculated. All data are plotted (each dot represents each individual) in the graph with boxplots showing quartiles. Asterisk indicates a significant difference compared to the Basal diet group in the Wilcoxon rank-sum test (B, P=3.56×10−3; C, P=8.33×10−3). There was no significant difference (n.s.) between the two groups in mass per egg (D, P=0.973). Atsushi Miyashita et al. Biology Open 2016;bio.019190 © 2016. Published by The Company of Biologists Ltd